Source:http://linkedlifedata.com/resource/pubmed/id/20946022
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2010-11-24
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| pubmed:abstractText |
A preparative column chromatographic method for isolation of carotenoids and chlorophylls from Gynostemma pentaphyllum, a traditional Chinese herb, was developed to evaluate their antiproliferative effects on the hepatoma cell Hep3B. An open column containing 70 g of magnesium oxide-diatomaceous earth (1:2.5, wt/wt) was used to elute carotenoid with 2% ethanol in ethyl acetate and chlorophyll with 50% ethanol in acetone. After high-performance liquid chromatography-mass spectrometry analysis, the carotenoid fraction was composed of all-trans- and cis-isomers of lutein, ?-carotene, and ?-carotene as well as epoxy-containing carotenoids, while the chlorophyll fraction consisted of chlorophylls a and b and their derivatives. Both carotenoid and chlorophyll fractions as well as lutein and chlorophyll a standards at 50-100 ?g/mL were effective against Hep3B cells with a dose-dependent response with the following order: carotenoid fraction?>?chlorophyll fraction?>?lutein?>?chlorophyll a. For all treatments, the cell cycle was arrested in the G?/G? phase, with Hep3B cells undergoing necrosis or apoptosis.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
|
| pubmed:issn |
1557-7600
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
13
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1431-42
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| pubmed:meshHeading |
pubmed-meshheading:20946022-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:20946022-Apoptosis,
pubmed-meshheading:20946022-Carcinoma, Hepatocellular,
pubmed-meshheading:20946022-Carotenoids,
pubmed-meshheading:20946022-Cell Line, Tumor,
pubmed-meshheading:20946022-Cell Proliferation,
pubmed-meshheading:20946022-Cell Survival,
pubmed-meshheading:20946022-Chlorophyll,
pubmed-meshheading:20946022-Chromatography, Liquid,
pubmed-meshheading:20946022-Drugs, Chinese Herbal,
pubmed-meshheading:20946022-G0 Phase,
pubmed-meshheading:20946022-G1 Phase,
pubmed-meshheading:20946022-Gynostemma,
pubmed-meshheading:20946022-Humans,
pubmed-meshheading:20946022-Inhibitory Concentration 50,
pubmed-meshheading:20946022-Liver Neoplasms,
pubmed-meshheading:20946022-Necrosis,
pubmed-meshheading:20946022-Plant Leaves,
pubmed-meshheading:20946022-Spectrometry, Mass, Electrospray Ionization
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Preparation of carotenoids and chlorophylls from Gynostemma pentaphyllum (Thunb.) Makino and their antiproliferation effect on hepatoma cell.
|
| pubmed:affiliation |
Department of Food Science, Fu Jen University, Taipei, Taiwan.
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| pubmed:publicationType |
Journal Article,
Comparative Study
|