Linked Life Data

20936903

Source:http://linkedlifedata.com/resource/pubmed/id/20936903

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-10-12
pubmed:abstractText
The oocyte capacity to rejuvenate a differentiated nucleus to restart the proper embryonic program has been highly conserved between vertebrate species. In view of the recent progress to induce pluripotency in somatic cells with stemness genes, we investigated the potential of oocyte genes to contribute to chromatin rearrangements in somatic cells. We selected conserved genes that are naturally expressed mainly in oocytes and that were susceptible to play a role in reprogramming during early embryogenesis. We induced their expression by transient transfection in HEK293?cells. We then assessed whether they had a global impact on epigenetic events such as histone core modifications, and also on transcription and expression of pluripotency-associated transcription factors. Nucleoplasmin 2 (NPM2), activation-induced cytidine deaminase (AICDA), and Geminin (GMNN) overexpression induced differences in histone core modifications (methylation and acetylation). AICDA and NPM2 also influenced RNA neosynthesis. NPM2, GMNN, and STELLA induced overexpression of well-known pluripotency transcription factors. Overall, AICDA, GMNN, NPM2, and STELLA influenced at least one of the aspects analyzed. Their potential could be useful in increasing the cell receptivity to pluripotency induction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
2152-4998
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
519-28
pubmed:meshHeading
pubmed-meshheading:20936903-Base Sequence, pubmed-meshheading:20936903-Cell Dedifferentiation, pubmed-meshheading:20936903-Cell Line, pubmed-meshheading:20936903-Cell Proliferation, pubmed-meshheading:20936903-Chromatin Assembly and Disassembly, pubmed-meshheading:20936903-DNA Primers, pubmed-meshheading:20936903-Epigenesis, Genetic, pubmed-meshheading:20936903-Female, pubmed-meshheading:20936903-Gene Expression Regulation, Developmental, pubmed-meshheading:20936903-Homeodomain Proteins, pubmed-meshheading:20936903-Humans, pubmed-meshheading:20936903-Octamer Transcription Factor-3, pubmed-meshheading:20936903-Oocytes, pubmed-meshheading:20936903-Pluripotent Stem Cells, pubmed-meshheading:20936903-RNA, Messenger, pubmed-meshheading:20936903-SOXB1 Transcription Factors, pubmed-meshheading:20936903-Transfection
pubmed:year
2010
pubmed:articleTitle
Investigating the potential of genes preferentially expressed in oocyte to induce chromatin remodeling in somatic cells.
pubmed:affiliation
Département des Sciences Animales, Université Laval, Québec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't