Linked Life Data

20924679

Source:http://linkedlifedata.com/resource/pubmed/id/20924679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-12-24
pubmed:abstractText
This study uses scanning acoustic microscopy (SAM) ultrasonic profilometry to determine acceptable vs. failed tissue engineered oral mucosa. Specifically, ex vivo-produced oral mucosal equivalents (EVPOMEs) under normal or thermally stressed culture conditions were scanned with the SAM operator blinded to the culture conditions. As seeded cells proliferate, they fill in and smooth out the surface irregularities; they then stratify and produce a keratinized protective upper layer. Some of these transformations could alter backscatter of ultrasonic signals and in the case of the thermally stressed cells, produce backscatter similar to an unseeded device. If non-invasive ultrasonic monitoring could be developed, then tissue cultivation could be adjusted to measure biological variations in the stratified surface. To create an EVPOME device, oral mucosa keratinocytes were seeded onto acellular cadaveric dermis. Two sets of EVPOMEs were cultured: one at physiological temperature 37 °C and the other at 43 °C. The specimens were imaged with SAM consisting of a single-element transducer: 61 MHz center frequency, 32 MHz bandwidth, 1.52 f#. Profilometry for the stressed and unseeded specimens showed higher surface irregularities compared to unstressed specimens. Elevated thermal stress retards cellular differentiation, increasing root mean square values; these results show that SAM can potentially monitor cell/tissue development.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-10413749, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-10765951, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-11805511, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-12079696, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-12188895, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-12729781, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-14745242, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-1701584, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-17257739, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-18461486, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-20379033, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-2321315, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-2529060, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-3891129, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-7124643, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-9140180, http://linkedlifedata.com/resource/pubmed/commentcorrection/20924679-9203490
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1521-6047
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
44-52
pubmed:dateRevised
2011-8-29
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Acoustic microscopy analyses to determine good vs. failed tissue engineered oral mucosa under normal or thermally stressed culture conditions.
pubmed:affiliation
Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Lurie BME Bldg., No. 2122, Ann Arbor, MI 48109, USA. fwinterr@umich.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural