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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2010-11-24
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pubmed:abstractText |
This study examined the role of focal adhesion kinase (FAK) as a mechanoreceptor in human periodontal ligament (hPDL) cells. hPDL cells were obtained from premolars extracted for orthodontic purposes. Mechanical stress was applied in a compressive manner (2 g/cm(2)) for various time durations (0.5, 2, 6, 24, 48 hrs) with or without a knockdown treatment for FAK. Compressive stimulation increased the level of phosphorylated FAK and prostaglandin E(2) production. The FAK-knockdown cells showed significantly lower prostaglandin E(2) levels than the control cells. Furthermore, compressive stress up-regulated cyclo-oxygenase-2 mRNA, whereas there were no changes observed in the FAK-knockdown cells. These results suggest that FAK regulates the production of prostaglandin E(2) via the transcriptional regulation of COX-2 mRNA in compressive stimulated PDL cells. The FAK-integrin complex plays a role in mechanoreception and mechanotransduction in hPDL cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
D
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1544-0591
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1444-9
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pubmed:meshHeading |
pubmed-meshheading:20924071-Biomechanics,
pubmed-meshheading:20924071-Blotting, Western,
pubmed-meshheading:20924071-Cell Survival,
pubmed-meshheading:20924071-Cells, Cultured,
pubmed-meshheading:20924071-Cyclooxygenase 2,
pubmed-meshheading:20924071-Dinoprostone,
pubmed-meshheading:20924071-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:20924071-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:20924071-Focal Adhesion Kinase 1,
pubmed-meshheading:20924071-Gene Knockdown Techniques,
pubmed-meshheading:20924071-Humans,
pubmed-meshheading:20924071-Mechanoreceptors,
pubmed-meshheading:20924071-Mechanotransduction, Cellular,
pubmed-meshheading:20924071-Periodontal Ligament,
pubmed-meshheading:20924071-Phosphorylation,
pubmed-meshheading:20924071-Protein Processing, Post-Translational,
pubmed-meshheading:20924071-RNA, Small Interfering,
pubmed-meshheading:20924071-Stress, Mechanical,
pubmed-meshheading:20924071-Time Factors,
pubmed-meshheading:20924071-Transcription, Genetic,
pubmed-meshheading:20924071-Up-Regulation
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pubmed:year |
2010
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pubmed:articleTitle |
FAK pathway regulates PGE? production in compressed periodontal ligament cells.
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pubmed:affiliation |
Department of Orthodontics, Kyung Hee University Dental College, Hoegi-dong 1, Dongdaemun-gu, Seoul 130–701, Korea.
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pubmed:publicationType |
Journal Article,
Comparative Study
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