Linked Life Data

20887853

Source:http://linkedlifedata.com/resource/pubmed/id/20887853

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-10-4
pubmed:abstractText
Aqueous biological samples must be "preserved" (stabilized) before they can be placed in the high vacuum of an electron microscope. Among the various approaches that have been developed, plunge freezing maintains the sample in the most native state and is therefore the method of choice when possible. Plunge freezing for standard electron cryomicroscopy applications proceeds by spreading the sample into a thin film across an EM grid and then rapidly submerging it in a cryogen (usually liquid ethane), but success depends critically on the properties of the grid and sample, the production of a uniformly thin film, the temperature and nature of the cryogen, and the plunging conditions. This chapter reviews plunge-freezing principles, techniques, instrumentation, common problems, and safety considerations.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1557-7988
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
481
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-82
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Plunge freezing for electron cryomicroscopy.
pubmed:affiliation
Division of Biology, California Institute of Technology, Pasadena, California, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural