|
rdf:type |
|
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lifeskim:mentions |
umls-concept:C0012632,
umls-concept:C0023272,
umls-concept:C0023276,
umls-concept:C0185125,
umls-concept:C0205210,
umls-concept:C0205234,
umls-concept:C0337112,
umls-concept:C0370003,
umls-concept:C1008763,
umls-concept:C1335439,
umls-concept:C1444754,
umls-concept:C1510438,
umls-concept:C1524075,
umls-concept:C1527148,
umls-concept:C1708096,
umls-concept:C1882417,
umls-concept:C2347026
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|
pubmed:issue |
2
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|
pubmed:dateCreated |
2010-9-17
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|
pubmed:abstractText |
Topoisomerase II gene of Leishmania genus was used to develop a molecular tool for detection and species differentiation of Leishmania from clinical samples. Identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases BstU1 and Taq1. Despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1879-0070
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pubmed:author |
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pubmed:copyrightInfo |
Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
152-8
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pubmed:meshHeading |
pubmed-meshheading:20846587-Base Sequence,
pubmed-meshheading:20846587-DNA, Protozoan,
pubmed-meshheading:20846587-DNA Topoisomerases, Type II,
pubmed-meshheading:20846587-Humans,
pubmed-meshheading:20846587-Leishmania,
pubmed-meshheading:20846587-Leishmania infantum,
pubmed-meshheading:20846587-Leishmania major,
pubmed-meshheading:20846587-Leishmaniasis,
pubmed-meshheading:20846587-Leishmaniasis, Cutaneous,
pubmed-meshheading:20846587-Molecular Diagnostic Techniques,
pubmed-meshheading:20846587-Polymerase Chain Reaction,
pubmed-meshheading:20846587-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:20846587-Sensitivity and Specificity,
pubmed-meshheading:20846587-Sequence Analysis, DNA,
pubmed-meshheading:20846587-Species Specificity,
pubmed-meshheading:20846587-Tunisia
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pubmed:year |
2010
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pubmed:articleTitle |
Development of a polymerase chain reaction-restriction fragment length polymorphism assay for Leishmania major/Leishmania killicki/Leishmania infantum discrimination from clinical samples, application in a Tunisian focus.
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pubmed:affiliation |
Laboratory of Parasitology-Mycology (99UR/08-05), Department of Clinical Biology B, Faculty of Pharmacy, Monastir, Tunisia. najoua.h@laposte.net
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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