Linked Life Data

2082185

Source:http://linkedlifedata.com/resource/pubmed/id/2082185

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1991-5-7
pubmed:abstractText
Human progesterone receptors (PR) are thought to comprise two naturally occurring hormone-binding proteins: 94-kDa A-receptors and 120-kDa B-receptors. In this paper we present evidence for a third human PR, an N-terminally truncated, 45- to 50-kDa species, termed the C-receptor. To determine the translational origin of B- and A-receptors we mapped the multiple messages that code for human PR by Northern blot analyses, using a series of oligonucleotides and cDNA fragment probes corresponding to different regions of the PR message. In addition to the six transcripts of 2.5, 3.2, 4.5, 5.2, 6.1, and 11.4 kilobases (kb) originally described, we found that the 11.4-kb species is a complex of four bands that we have termed I-IV. Analysis of poly(A)+ RNA derived from T47Dv human breast cancer cells using a variety of 5'-specific probes has identified three separate structural classes of human PR transcripts, indicating extensive 5'-termini heterogeneity. Class A messages, the 2.5- and 5.2-kb species, lack the sequences surrounding AUGB (codon 1), which is the translation initiation site for B-receptors, but contain AUGA (codon 165), the initiation site for A-receptors, and, therefore, potentially encode only the latter. Class B messages, consisting of the 3.2-, 4.5-, and 6.1-kb species as well as bands I and II of the 11.4-kb complex contain both AUGB and AUGA and could encode both receptor forms.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1833-40
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
5'-Heterogeneity in human progesterone receptor transcripts predicts a new amino-terminal truncated "C"-receptor and unique A-receptor messages.
pubmed:affiliation
Department of Medicine, University of Colorado Health Sciences Center, Denver 80262.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't