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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2010-9-8
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pubmed:abstractText |
Neutrophils have key roles in modulating the immune response. We present a robust methodology for rapidly isolating neutrophils directly from whole blood with 'on-chip' processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulation experiment and by comparison with standard bulk isolation methodologies. Last, we implement this tool as part of a near-patient blood processing system within a multi-center clinical study of the immune response to severe trauma and burn injury. The preliminary results from a small cohort of subjects in our study and healthy controls show a unique time-dependent gene expression pattern clearly demonstrating the ability of this tool to discriminate temporal transcriptional events of neutrophils within a clinical setting.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/P01 HG000205,
http://linkedlifedata.com/resource/pubmed/grant/P01 HG000205-14,
http://linkedlifedata.com/resource/pubmed/grant/P01 HG000205-16,
http://linkedlifedata.com/resource/pubmed/grant/P41 EB-002503,
http://linkedlifedata.com/resource/pubmed/grant/P41 EB002503-05,
http://linkedlifedata.com/resource/pubmed/grant/P41 EB002503-07,
http://linkedlifedata.com/resource/pubmed/grant/P41 RR018522-05,
http://linkedlifedata.com/resource/pubmed/grant/P41 RR018522-06,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM036214-18,
http://linkedlifedata.com/resource/pubmed/grant/R01-GM-36214,
http://linkedlifedata.com/resource/pubmed/grant/T32 GM-007035-32,
http://linkedlifedata.com/resource/pubmed/grant/T32 GM007035-32,
http://linkedlifedata.com/resource/pubmed/grant/U54 GM-062119,
http://linkedlifedata.com/resource/pubmed/grant/U54 GM062119-09,
http://linkedlifedata.com/resource/pubmed/grant/U54 GM062119-10
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-11134512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-11309499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-11943771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-12495940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-12626596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-14634056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-1545121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-15514007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-15613496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-15781863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-15800882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16136080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16166516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16204629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16237112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16335993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16408053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16429408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16446425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16498448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16515398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16609939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-16887931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-17032758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-17255552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-17338817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-17414933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-18097410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-18453102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-18453552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-18820680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-7880385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-8335942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-8335946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-9129050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20802500-9704051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1546-170X
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pubmed:author |
pubmed-author:BankeyPaul EPE,
pubmed-author:BrownsteinBernard HBH,
pubmed-author:CampDavid GDG2nd,
pubmed-author:DavisRonald WRW,
pubmed-author:DeAsitA,
pubmed-author:FaganShawn PSP,
pubmed-author:GovermanJeremyJ,
pubmed-author:Inflammation and the Host Response to Injury Collaborative Research...,
pubmed-author:IrimiaDanielD,
pubmed-author:KotzKenneth TKT,
pubmed-author:Miller-GrazianoCarolC,
pubmed-author:MindrinosMichael NMN,
pubmed-author:MoldawerLyle LLL,
pubmed-author:QianWei-JunWJ,
pubmed-author:RosenbachAlan EAE,
pubmed-author:RussomAmanA,
pubmed-author:SmithRichard DRD,
pubmed-author:TompkinsRonald GRG,
pubmed-author:TonerMehmetM,
pubmed-author:WarnerElizabeth AEA,
pubmed-author:WilhelmyJulieJ,
pubmed-author:XiaoWenzongW,
pubmed-author:XuWeihongW,
pubmed-author:de CortinezY JYJ
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pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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