Linked Life Data

20730136

Source:http://linkedlifedata.com/resource/pubmed/id/20730136

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2010-9-21
pubmed:abstractText
Escherichia coli RNA polymerase is a multi-subunit enzyme containing ?(2)??'??, which transcribes DNA template to intermediate RNA product in a sequence specific manner. Although most of the subunits are essential for its function, the smallest subunit ? (average molecular mass ? 10,105 Da) can be deleted without affecting bacterial growth. Creating a mutant of the ? subunit can aid in improving the understanding of its role. Sequencing of rpoZ gene that codes for ? subunit from a mutant variant suggested a substitution mutation at position 60 of the protein: asparagine (N) ? aspartic acid (D). This mutation was verified at the protein level by following a typical mass spectrometry (MS) based bottom-up proteomic approach. Characterization of in-gel trypsin digested samples by reverse phase liquid chromatography (LC) coupled to electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) enabled in ascertaining this mutation. Electron transfer dissociation (ETD) of triply charged [(M + 3H)(3+)] tryptic peptides (residues [53-67]), EIEEGLINNQILDVR from wild-type and EIEEGLIDNQILDVR from mutant, facilitated in unambiguously determining the site of mutation at residue 60.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1364-5528
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2723-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Identifying N60D mutation in ? subunit of Escherichia coli RNA polymerase by bottom-up proteomic approach.
pubmed:affiliation
Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bengaluru, 560064, India. sabareesh@jncasr.ac.in
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't