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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2010-8-10
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pubmed:abstractText |
Zernike phase contrast cryo-electron microscopy (ZPC-cryoEM) is an emerging technique that is capable of producing higher image contrast than conventional cryoEM. By combining this technique with advanced image processing methods, we achieved subnanometer resolution for two biological specimens: 2D bacteriorhodopsin crystal and epsilon15 bacteriophage. For an asymmetric reconstruction of epsilon15 bacteriophage, ZPC-cryoEM can reduce the required amount of data by a factor of approximately 3, compared with conventional cryoEM. The reconstruction was carried out to 13 A resolution without the need to correct the contrast transfer function. New structural features at the portal vertex of the epsilon15 bacteriophage are revealed in this reconstruction. Using ZPC cryo-electron tomography (ZPC-cryoET), a similar level of data reduction and higher resolution structures of epsilon15 bacteriophage can be obtained relative to conventional cryoET. These results show quantitatively the benefits of ZPC-cryoEM and ZPC-cryoET for structural determinations of macromolecular machines at nanometer and subnanometer resolutions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-10600563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-11356062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-12781660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-14568533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-15193640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-15264254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-15603052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-16452981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-16949755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-17055742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-17079082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-17698370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-17967545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-18082423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-18299206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-18305544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-18313941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-18514542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-19157711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-20119415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-20152149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-20338243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-20543830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-3043536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-3188279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-4592808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-7568675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696391-8742726
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1878-4186
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
11
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
903-12
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pubmed:dateRevised |
2011-8-12
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pubmed:meshHeading |
pubmed-meshheading:20696391-Bacteriophages,
pubmed-meshheading:20696391-Bacteriorhodopsins,
pubmed-meshheading:20696391-Cryoelectron Microscopy,
pubmed-meshheading:20696391-Crystallography,
pubmed-meshheading:20696391-Electron Microscope Tomography,
pubmed-meshheading:20696391-Epsilonproteobacteria,
pubmed-meshheading:20696391-Image Processing, Computer-Assisted,
pubmed-meshheading:20696391-Microscopy, Phase-Contrast,
pubmed-meshheading:20696391-Models, Molecular
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pubmed:year |
2010
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pubmed:articleTitle |
Zernike phase contrast cryo-electron microscopy and tomography for structure determination at nanometer and subnanometer resolutions.
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pubmed:affiliation |
National Center for Macromolecular Imaging, Verna and Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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