Linked Life Data

20696009

Source:http://linkedlifedata.com/resource/pubmed/id/20696009

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-10-19
pubmed:abstractText
• SOS1 is an Na(+)/H(+) antiporter that plays a central role in Na(+) tolerance in land plants. SOS1 mediation of Na(+) efflux has been studied in plasma-membrane vesicles and deduced from the SOS1 suppression of the Na(+) sensitivity of yeast mutants defective in Na(+) -efflux. However, SOS1-mediated Na(+) efflux has not been characterized in either plant or yeast cells. Here, we use Physcomitrella patens to investigate the function of SOS1 in planta. • In P. patens, a nonvascular plant in which the study of ion cellular fluxes is technically simple, the existence of two SOS1 genes suggests that the Na(+) efflux remaining after the deletion of the ENA1 ATPase is mediated by a SOS1 system. Therefore, we cloned the P. patens SOS1 and SOS1B genes (PpSOS1 and PpSOS1B, respectively) and complementary DNAs, and constructed the Pp?sos1 and Pp?ena1/Pp?sos1 deletion lines by gene targeting. • Comparison of wild-type, and Pp?sos1 and Pp?ena1/Pp?sos1 mutant lines revealed that PpSOS1 is crucial for Na(+) efflux and that the Pp?sos1 line, and especially the Pp?ena1/Pp?sos1 lines, showed excessive Na(+) accumulation and Na(+)-triggered cell death. The Pp?sos1 and Pp?ena1/Pp?sos1 lines showed impaired high-affinity K(+) uptake. • Our data support the hypothesis that PpSOS1 mediates cellular Na(+) efflux and that PpSOS1 enhances K(+) uptake by an indirect effect.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1469-8137
pubmed:author
pubmed:copyrightInfo
© The Authors (2010). Journal compilation © New Phytologist Trust (2010).
pubmed:issnType
Electronic
pubmed:volume
188
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
750-61
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
The SOS1 transporter of Physcomitrella patens mediates sodium efflux in planta.
pubmed:affiliation
Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't