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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1991-8-9
|
| pubmed:abstractText |
Insulin-like growth factor-I (IGF-I) and IGF-II receptors have previously been demonstrated on membranes prepared from human somatotrophinomas. IGF-I has been shown to have a variable effect on GH secretion by these tumours in vitro. The effects of purified IGF-II on GH secretion have not been described. We have studied the direct actions of human recombinant IGF-II on GH release from eight somatotrophinomas cultured in vitro. Somatotrophinoma cells were cultured as monolayers at a density of 10(5) cells/0.5 ml. Treatment with IGF-II for 4 and 24 h resulted in discrete inhibitory effects on GH release from two tumours (tumour 5: 4 h, IGF-II 0.5 nmol/l; tumour 2; 24 h, IGF-II 1 nmol/l). Treatment with IGF-II for 24 h resulted in significant inhibitory effects on GH release from one tumour over a range of concentrations tested (IGF-II 0.5-10 nmol/l). Addition of human GH-releasing factor (hGRF) (1-44) (20 nmol/l) for 4 and 24 h resulted in stimulation of GH release by five tumours. Two tumours demonstrated significant inhibitory effects of IGF-II on GRF-stimulated GH release (tumour 2: 24 h, IGF-II 1-5 nmol/l; tumour 3; 4 h, IGF-II 5 nmol/l; 24 h, IGF-II 0.5-50 nmol/l). These data emphasize the heterogeneity of somatotrophinomas in terms of their response to modulators of GH secretion. IGF-II does not appear to have a modulatory role on GH release by most somatotrophinomas.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-0795
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
129
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
447-51
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2066699-Adult,
pubmed-meshheading:2066699-Aged,
pubmed-meshheading:2066699-Female,
pubmed-meshheading:2066699-Growth Hormone,
pubmed-meshheading:2066699-Growth Hormone-Releasing Hormone,
pubmed-meshheading:2066699-Humans,
pubmed-meshheading:2066699-Insulin-Like Growth Factor II,
pubmed-meshheading:2066699-Male,
pubmed-meshheading:2066699-Middle Aged,
pubmed-meshheading:2066699-Pituitary Neoplasms,
pubmed-meshheading:2066699-Time Factors,
pubmed-meshheading:2066699-Tumor Cells, Cultured
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Effects of insulin-like growth factor-II on growth hormone release from human somatotrophinoma cells in vitro.
|
| pubmed:affiliation |
Department of Medicine, University of Newcastle upon Tyne.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|