|
rdf:type |
|
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lifeskim:mentions |
umls-concept:C0032520,
umls-concept:C0205134,
umls-concept:C0205345,
umls-concept:C0370003,
umls-concept:C0549193,
umls-concept:C0680844,
umls-concept:C0681916,
umls-concept:C0750572,
umls-concept:C1514811,
umls-concept:C1561577,
umls-concept:C1706462,
umls-concept:C2347026,
umls-concept:C2587213
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pubmed:issue |
7
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pubmed:dateCreated |
2010-7-27
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pubmed:abstractText |
Real-Time quantitative PCR is an important tool in research and clinical settings. Here, we describe two new approaches that broaden the scope of real-time quantitative PCR; namely, run-internal mini standard curves (RIMS) and direct real-time relative quantitative PCR (drqPCR). RIMS are an efficient alternative to traditional standard curves and provide both run-specific and target-specific estimates of PCR parameters. The drqPCR enables direct estimation of target ratios without reference to conventional control samples.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-11013345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-11328886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-11846609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-12184808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-12907745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-15249599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-16060372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-16351727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-17291332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-18074404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-18474036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-18546601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-19237396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-7764001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20661435-8449504
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
1932-6203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e11723
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pubmed:meshHeading |
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pubmed:year |
2010
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pubmed:articleTitle |
Real-time relative qPCR without reference to control samples and estimation of run-specific PCR parameters from run-internal mini-standard curves.
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pubmed:affiliation |
Department of Clinical Immunology, University Hospital of Aarhus, Skejby, Denmark. jejensen@rm.dk
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pubmed:publicationType |
Journal Article
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