Source:http://linkedlifedata.com/resource/pubmed/id/20640151
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2010-7-19
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pubmed:abstractText |
Tanshinone I (Tan-I) is a diterpene quinone extracted from the traditional herbal medicine Salvia miltiorrhiza Bunge. Recently, Tan-I has been reported to have anti-tumor effects. In this study, we investigated the growth inhibition and apoptosis inducing effects of Tan-I on three kinds of monocytic leukemia cells (U937, THP-1 and SHI 1). Cell viability was measured by MTT assay. Cell apoptosis was assessed by flow cytometry (FCM) and AnnexinV/PI staining. Reverse transcriptase polymerase chain reaction (RT-PCR) and PCR-enzyme-linked immunosorbent assay (ELISA) were used to detect human telomerase reverse transcriptase (hTERT) expression and telomerase activity before and after apoptosis. The activity of caspase-3 was determined by Caspase colorimetric assay kit and Western blot analysis. Expression of the anti-apoptotic gene Survivin was assayed by Western blot and Real-time RT-PCR using the ABI PRISM 7500 Sequence Detection System. The results revealed that Tan-I could inhibit the growth of these three kinds of leukemia cells and cause apoptosis in a time- and dose-dependent manner. After treatment by Tan-I for 48 h, Western blotting showed cleavage of the caspase-3 zymogen protein with the appearance of its 17-kD subunit, and a 89-kD cleavage product of poly (ADP-ribose) polymerase (PARP), a known substrate of caspase-3, was also found clearly. The expression of hTERT mRNA as well as activity of telomerase were decreased concurrently in a dose-dependent manner. Moreover, Real-time RT-PCR and Western blot revealed a significant down-regulation of Survivin. We therefore conclude that the induction of apoptosis by Tan-I in monocytic leukemia U937 THP-1 and SHI 1 cells is highly correlated with activation of caspase-3 and decreasing of hTERT mRNA expression and telomerase activity as well as down-regulation of Survivin expression. To our knowledge, this is the first report about the effects of Tan-I on monocytic leukemia cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-12604411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-12646945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-12708468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-12760856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-14594517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-14649329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-14749900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-16291709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-16364206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-16652154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-17328950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-18414191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-18448098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-18449204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-18493662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-18584350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-18695877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-18949381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-19001436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-19559538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20640151-20166933
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pubmed:language |
eng
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pubmed:journal | |
pubmed:status |
PubMed-not-MEDLINE
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pubmed:issn |
1422-0067
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pubmed:author |
pubmed-author:FanRui-FangRF,
pubmed-author:FangZhi-GangZG,
pubmed-author:GuanWei-BingWB,
pubmed-author:HuangHe-QingHQ,
pubmed-author:HuangRen-WeiRW,
pubmed-author:LinDong-JunDJ,
pubmed-author:LiuJia-JunJJ,
pubmed-author:LiuPei-QingPQ,
pubmed-author:LiuXiao-DanXD,
pubmed-author:XiaoRuo-ZhiRZ,
pubmed-author:YangHong-ZhiHZ,
pubmed-author:ZhangYongY
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pubmed:issnType |
Electronic
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2267-80
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pubmed:year |
2010
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pubmed:articleTitle |
Down-regulation of telomerase activity and activation of caspase-3 are responsible for tanshinone I-induced apoptosis in monocyte leukemia cells in vitro.
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pubmed:affiliation |
Hematological Department & Institute, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China; E-Mails: lenovo381@126.com (X.-D.L.); xfangcn@163.com (R.-F.F.); fzg92@163.com (Z.-G.F.); dongjunlin0168@163.com (D.-J.L.); ruozhi_xiao@yahoo.com (R.-Z.X.); huangrw56@163.com (R.-W.H.).
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pubmed:publicationType |
Journal Article
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