Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2010-7-19
pubmed:abstractText
The pneumonic lesions and mortality caused by Mannheimia haemolytica in bighorn sheep (BHS; Ovis canadensis) are more severe than those in the related species, domestic sheep (DS; Ovis aries), under both natural and experimental conditions. Leukotoxin (Lkt) and lipopolysaccharide (LPS) are the most important virulence factors of this organism. One hallmark of pathogenesis of pneumonia is the influx of polymorphonuclear leukocytes (PMNs) into the lungs. Lkt-induced cytolysis of PMNs results in the release of cytotoxic compounds capable of damaging lung tissue. Interleukin-8 (IL-8) is a potent PMN chemoattractant. The objective of the present study was to determine if there is differential expression of IL-8 by the macrophages and PMNs of BHS and DS in response to M. haemolytica. Macrophages and PMNs of BHS and DS were stimulated with heat-killed M. haemolytica or LPS. IL-8 expression by the cells was measured by enzyme-linked immunosorbent assays and real-time reverse transcription-PCR (RT-PCR). The PMNs of BHS expressed severalfold higher levels of IL-8 than those of DS upon stimulation. Lesional lung tissue of M. haemolytica-infected BHS contained significantly higher levels of IL-8 than nonlesional tissue. The bronchoalveolar lavage (BAL) fluid of infected BHS also contained higher levels of IL-8 than that of infected DS. Depletion of IL-8 reduced migration of PMNs toward BAL fluid by approximately 50%, indicating that IL-8 is integral to PMN recruitment to the lung during M. haemolytica infection. Excessive production of IL-8, enhanced recruitment of PMNs, and PMN lysis by Lkt are likely responsible for the severity of the lung lesions in M. haemolytica-infected BHS.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-10343061, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-10414364, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-10481101, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-11355660, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-11399141, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-11768129, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-11911574, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-12654776, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-1321532, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-15984339, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-17156740, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-1753879, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-18689665, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-18771862, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-1972901, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-2463967, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-2707855, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-2914876, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-3196974, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-3512509, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-3629916, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-4073635, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-7018660, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-7047767, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-7294474, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-7476090, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-7822000, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-8028096, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-8445788, http://linkedlifedata.com/resource/pubmed/commentcorrection/20515932-9539366
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1098-5522
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3578-84
pubmed:dateRevised
2011-7-22
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infection.
pubmed:affiliation
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't