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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2010-7-27
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pubmed:abstractText |
As genetically engineered mutant mice deficient in single genes are usually generated on a C57BL/6 background, to study mast cell trafficking in mutant mice, we initially investigated whether mast cells accumulated in bronchi in C57BL/6 mice challenged with OVA allergen acutely or chronically for 1 to 3 months. The total number of bronchial mast cells were quantitated using toluidine blue staining in airways of different sizes, i.e. , small (<90 microm), medium (90-155 microm), or large (>150 microm) airways. Non-OVA challenged and acute OVA challenged mice (C57BL/6 and BALB/c) had no detectable bronchial mast cells. Chronic OVA challenge in BALB/c mice for 1 or 3 months induced a significant increase in the number of bronchial mast cells in small-, medium-, and large-sized airways but minimal change in the number of bronchial mast cells in C57BL/6 mice. Both BALB/c and C57BL/6 mice developed significant lung eosinophilia following acute or chronic OVA challenge. Studies of IL-9-deficient mice on a BALB/c background demonstrated a significant increase in the number of bronchial mast cells in IL-9-deficient mice suggesting that IL-9 was not required for the bronchial accumulation of mast cells. Overall, these studies demonstrate that the chronic OVA challenge protocol we have utilized in BALB/c mice provides a model to study the mechanism of bronchial mast cell accumulation and that bronchial mast cell accumulation in chronic OVA challenged mice is independent of IL-9 in this model.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-10607813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-10725747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-10808163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-11781365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-12037156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-12857720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-12859460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-14568966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-14617508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-14966564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-16710480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-16750987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-1698556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-1717532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-17379701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-17471178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-18031566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-18483499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-18490489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-19032363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-19218812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-19224206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-19766085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-19783672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-3090545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-6198393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-8450046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-9558107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20480160-9763610
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1432-1211
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
499-506
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pubmed:dateRevised |
2010-9-28
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pubmed:meshHeading |
pubmed-meshheading:20480160-Allergens,
pubmed-meshheading:20480160-Animals,
pubmed-meshheading:20480160-Asthma,
pubmed-meshheading:20480160-Bronchi,
pubmed-meshheading:20480160-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:20480160-Disease Models, Animal,
pubmed-meshheading:20480160-Female,
pubmed-meshheading:20480160-Interleukin-9,
pubmed-meshheading:20480160-Mast Cells,
pubmed-meshheading:20480160-Mice,
pubmed-meshheading:20480160-Mice, Inbred BALB C,
pubmed-meshheading:20480160-Mice, Inbred C57BL,
pubmed-meshheading:20480160-Mice, Knockout,
pubmed-meshheading:20480160-Ovalbumin,
pubmed-meshheading:20480160-Pulmonary Eosinophilia,
pubmed-meshheading:20480160-Species Specificity
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pubmed:year |
2010
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pubmed:articleTitle |
Chronic allergen challenge induces bronchial mast cell accumulation in BALB/c but not C57BL/6 mice and is independent of IL-9.
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pubmed:affiliation |
Department of Medicine, University of California San Diego, San Diego, CA, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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