Linked Life Data

20433967

Source:http://linkedlifedata.com/resource/pubmed/id/20433967

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-5-3
pubmed:abstractText
A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) method has been developed for detection of proline. The molecularly imprinted polymer microspheres were synthesized using precipitation polymerization with hydroxyproline, a structural analogue, as the template. Polymer microspheres were immobilized in microtiter plates (96 wells) which selectively adsorbed the analyte (dansyl-proline). After washing, the bound fraction was quantified based on peroxyoxalate chemiluminescence (PO-CL) reaction enhanced by imidazole. The cavity of MIP synthesized with hydroxyproline as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-proline. Under the optimum conditions, the relative CL intensity has a linear relationship with the concentration of proline in the range of 1x10(-6) mol L(-1) to 4x10(-5) mol L(-1), with a limit of detection 3x10(-7) mol L(-1) (3sigma). The relative standard deviation (R.S.D.) for proline (1x10(-6) mol L(-1), n=7) was 3.7%. The MIP-CL method can become a useful analytical technology for determination of proline in real sample.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-4324
pubmed:author
pubmed:copyrightInfo
Crown Copyright 2010. Published by Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
666
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
70-5
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of proline.
pubmed:affiliation
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't