Linked Life Data

20430751

Source:http://linkedlifedata.com/resource/pubmed/id/20430751

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2010-4-30
pubmed:abstractText
The Drosophila anterior-posterior (AP) axis is determined by the polarisation of the stage 9 oocyte and the subsequent localisation of bicoid and oskar mRNAs to opposite poles of the cell. Oocyte polarity has been proposed to depend on the same PAR proteins that generate AP polarity in C. elegans, with a complex of Bazooka (Baz; Par-3), Par-6 and aPKC marking the anterior and lateral cortex, and Par-1 defining the posterior. The function of the Baz complex in oocyte polarity has remained unclear, however, because although baz-null mutants block oocyte determination, egg chambers that escape this early arrest usually develop normal polarity at stage 9. Here, we characterise a baz allele that produces a penetrant polarity phenotype at stage 9 without affecting oocyte determination, demonstrating that Baz is essential for axis formation. The dynamics of Baz, Par-6 and Par-1 localisation in the oocyte indicate that the axis is not polarised by a cortical contraction as in C. elegans, and instead suggest that repolarisation of the oocyte is triggered by posterior inactivation of aPKC or activation of Par-1. This initial asymmetry is then reinforced by mutual inhibition between the anterior Baz complex and posterior Par-1 and Lgl. Finally, we show that mutation of the aPKC phosphorylation site in Par-1 results in the uniform cortical localisation of Par-1 and the loss of cortical microtubules. Since non-phosphorylatable Par-1 is epistatic to uninhibitable Baz, Par-1 seems to function downstream of the other PAR proteins to polarize the oocyte microtubule cytoskeleton.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1477-9129
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1765-73
pubmed:dateRevised
2010-11-22
pubmed:meshHeading
pubmed-meshheading:20430751-Alleles, pubmed-meshheading:20430751-Animals, pubmed-meshheading:20430751-Animals, Genetically Modified, pubmed-meshheading:20430751-Body Patterning, pubmed-meshheading:20430751-Cell Nucleus, pubmed-meshheading:20430751-Cell Polarity, pubmed-meshheading:20430751-Cytoskeleton, pubmed-meshheading:20430751-Drosophila, pubmed-meshheading:20430751-Drosophila Proteins, pubmed-meshheading:20430751-Embryo, Nonmammalian, pubmed-meshheading:20430751-Female, pubmed-meshheading:20430751-Gene Expression Regulation, Developmental, pubmed-meshheading:20430751-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:20430751-Microtubules, pubmed-meshheading:20430751-Phosphorylation, pubmed-meshheading:20430751-Protein Kinase C, pubmed-meshheading:20430751-Protein-Serine-Threonine Kinases, pubmed-meshheading:20430751-Tissue Distribution, pubmed-meshheading:20430751-Tumor Suppressor Proteins
pubmed:year
2010
pubmed:articleTitle
Bazooka is required for polarisation of the Drosophila anterior-posterior axis.
pubmed:affiliation
The Gurdon Institute and Department of Genetics, University of Cambridge, Cambridge, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't