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pubmed-article:20430476pubmed:abstractTextIn previous research, iron-deficiency symptoms in peanut (Arachis hypgaea) were alleviated during anthesis by intercropping with maize. This benefit was associated with increased phytosiderophore secretion by maize and increased Fe(III)-chelate reductase activity by peanut. In the present study, we isolated the full-length cDNA of AhIRT1 (iron-regulated transporter 1) from peanut and characterized how iron deficiency and intercropping affected its iron-transporting ability. Functional complementation with AhIRT1 restored normal growth of the yeast mutant fet3fet4 (defective in both high- and low-affinity iron-uptake systems) under iron-deficiency conditions. Based on transient expression analysis, AhIRT1 was determined to be a membrane protein, which was consistent with a function in iron uptake. In peanut, transcript levels of AhIRT1 increased in both root and shoot under iron-deficiency conditions. In a pot experiment, AhIRT1 transcript levels in intercropped peanut were 10 times greater during anthesis than pre-anthesis, and transcript levels during anthesis were 40% greater in intercropped than in monocropped peanut.lld:pubmed
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pubmed-article:20430476pubmed:authorpubmed-author:LiJingJlld:pubmed
pubmed-article:20430476pubmed:authorpubmed-author:DingHongHlld:pubmed
pubmed-article:20430476pubmed:authorpubmed-author:ZhangFusuoFlld:pubmed
pubmed-article:20430476pubmed:authorpubmed-author:ZhaoMengMlld:pubmed
pubmed-article:20430476pubmed:authorpubmed-author:LiWen-XueWXlld:pubmed
pubmed-article:20430476pubmed:authorpubmed-author:DuanLihongLlld:pubmed
pubmed-article:20430476pubmed:authorpubmed-author:YanHuifengHlld:pubmed
pubmed-article:20430476pubmed:copyrightInfoCopyright 2010 Elsevier GmbH. All rights reserved.lld:pubmed
pubmed-article:20430476pubmed:issnTypeElectroniclld:pubmed
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pubmed-article:20430476pubmed:year2010lld:pubmed
pubmed-article:20430476pubmed:articleTitleCloning and functional analysis of the peanut iron transporter AhIRT1 during iron deficiency stress and intercropping with maize.lld:pubmed
pubmed-article:20430476pubmed:affiliationKey Laboratory of Plant and Soil Interactions, Ministry of Education, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China.lld:pubmed
pubmed-article:20430476pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20430476pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed