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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2010-7-15
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pubmed:abstractText |
In previous research, iron-deficiency symptoms in peanut (Arachis hypgaea) were alleviated during anthesis by intercropping with maize. This benefit was associated with increased phytosiderophore secretion by maize and increased Fe(III)-chelate reductase activity by peanut. In the present study, we isolated the full-length cDNA of AhIRT1 (iron-regulated transporter 1) from peanut and characterized how iron deficiency and intercropping affected its iron-transporting ability. Functional complementation with AhIRT1 restored normal growth of the yeast mutant fet3fet4 (defective in both high- and low-affinity iron-uptake systems) under iron-deficiency conditions. Based on transient expression analysis, AhIRT1 was determined to be a membrane protein, which was consistent with a function in iron uptake. In peanut, transcript levels of AhIRT1 increased in both root and shoot under iron-deficiency conditions. In a pot experiment, AhIRT1 transcript levels in intercropped peanut were 10 times greater during anthesis than pre-anthesis, and transcript levels during anthesis were 40% greater in intercropped than in monocropped peanut.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1618-1328
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Elsevier GmbH. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
167
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
996-1002
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pubmed:meshHeading |
pubmed-meshheading:20430476-Agriculture,
pubmed-meshheading:20430476-Amino Acid Sequence,
pubmed-meshheading:20430476-Arachis hypogaea,
pubmed-meshheading:20430476-Biological Transport,
pubmed-meshheading:20430476-Biomass,
pubmed-meshheading:20430476-Cloning, Molecular,
pubmed-meshheading:20430476-DNA, Plant,
pubmed-meshheading:20430476-Gene Expression Regulation, Plant,
pubmed-meshheading:20430476-Genetic Complementation Test,
pubmed-meshheading:20430476-Iron,
pubmed-meshheading:20430476-Membrane Proteins,
pubmed-meshheading:20430476-Molecular Sequence Data,
pubmed-meshheading:20430476-Mutation,
pubmed-meshheading:20430476-Plant Proteins,
pubmed-meshheading:20430476-RNA, Messenger,
pubmed-meshheading:20430476-RNA, Plant,
pubmed-meshheading:20430476-Recombinant Fusion Proteins,
pubmed-meshheading:20430476-Saccharomyces cerevisiae,
pubmed-meshheading:20430476-Sequence Alignment,
pubmed-meshheading:20430476-Sequence Analysis, DNA,
pubmed-meshheading:20430476-Stress, Physiological,
pubmed-meshheading:20430476-Zea mays
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pubmed:year |
2010
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pubmed:articleTitle |
Cloning and functional analysis of the peanut iron transporter AhIRT1 during iron deficiency stress and intercropping with maize.
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pubmed:affiliation |
Key Laboratory of Plant and Soil Interactions, Ministry of Education, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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