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pubmed-article:20416126pubmed:abstractTextThe objective of this study was to assess the proteolytic activity of Fasciola hepatica cathepsins in liver sections from mice vaccinated with phage clones of cathepsin L mimotopes, using the film in situ zymography technique. Female BALB/c mice were immunized three times with 2.5 x 10¹¹ phage particles without adjuvant. Animals vaccinated with phage clones produced high titres of anti-mimotope antibodies and a significant reduction in fluke burden was observed following challenge with metacercariae of F. hepatica. The proteolytic activity in hepatic tissue was reduced after the immunization with phage clones.lld:pubmed
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pubmed-article:20416126pubmed:articleTitleProteolytic activity in Fasciola hepatica is reduced by the administration of cathepsin L mimotopes.lld:pubmed
pubmed-article:20416126pubmed:affiliationLaboratorio de Genética y Reproducción, Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, 4 Sur 304 Col. Centro, C.P. 75482, Tecamachalco Puebla, México. abel.villa@gmail.comlld:pubmed
pubmed-article:20416126pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20416126pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed