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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2011-1-28
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pubmed:abstractText |
The objective of this study was to assess the proteolytic activity of Fasciola hepatica cathepsins in liver sections from mice vaccinated with phage clones of cathepsin L mimotopes, using the film in situ zymography technique. Female BALB/c mice were immunized three times with 2.5 x 10¹¹ phage particles without adjuvant. Animals vaccinated with phage clones produced high titres of anti-mimotope antibodies and a significant reduction in fluke burden was observed following challenge with metacercariae of F. hepatica. The proteolytic activity in hepatic tissue was reduced after the immunization with phage clones.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1475-2697
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
51-5
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pubmed:meshHeading |
pubmed-meshheading:20416126-Amino Acid Sequence,
pubmed-meshheading:20416126-Animals,
pubmed-meshheading:20416126-Antibodies, Helminth,
pubmed-meshheading:20416126-Bacteriophage M13,
pubmed-meshheading:20416126-Cathepsin L,
pubmed-meshheading:20416126-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:20416126-Epitopes,
pubmed-meshheading:20416126-Fasciola hepatica,
pubmed-meshheading:20416126-Fascioliasis,
pubmed-meshheading:20416126-Female,
pubmed-meshheading:20416126-Immunization,
pubmed-meshheading:20416126-Liver,
pubmed-meshheading:20416126-Mice,
pubmed-meshheading:20416126-Mice, Inbred BALB C,
pubmed-meshheading:20416126-Molecular Mimicry,
pubmed-meshheading:20416126-Peptide Library,
pubmed-meshheading:20416126-Vaccination,
pubmed-meshheading:20416126-Vaccines
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pubmed:year |
2011
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pubmed:articleTitle |
Proteolytic activity in Fasciola hepatica is reduced by the administration of cathepsin L mimotopes.
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pubmed:affiliation |
Laboratorio de Genética y Reproducción, Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, 4 Sur 304 Col. Centro, C.P. 75482, Tecamachalco Puebla, México. abel.villa@gmail.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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