Source:http://linkedlifedata.com/resource/pubmed/id/20383584
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2010-12-2
|
| pubmed:abstractText |
Bone marrow-derived mesenchymal stem cells (BMSCs) have been demonstrated to be a promising cell sources for cardiac regeneration. Poor survival rate of transplanted BMSCs in infarcted myocardium attenuated its clinical application. It's reported that stromal-derived factor-1 (SDF-1) could protect progenitor cells including endothelial progenitor cells and embryonic stem cells from apoptosis. But little is known whether SDF-1? protein has the same protective effects on BMSCs under conditions of hypoxia and serum deprivation (hypoxia/SD). In present study, we verified that SDF-1? (0.50-2.0 ?g/ml) inhibited hypoxia/SD induced apoptosis of BMSCs through mitochondrial pathway. After administration of SDF-1?, the loss of mitochondrial membrane potential and cytochrome c released from mitochondria to cytosol were significantly inhibited, and caspase 3 activity also declined. Furthermore, the effect of SDF-1? on mitochondrial pathway was neutralized by using PI3K inhibitor (Wortmannin) and ERK1/2 inhibitor (U0126). Our observations suggested that SDF-1? inhibits hypoxia/SD induced BMSCs apoptosis through PI3K/Akt and ERK1/2 signaling pathways. These data also imply that the anti-apoptotic effect mediated by SDF-1? may enhance cell survival after cell transplantation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
1573-4978
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
9-16
|
| pubmed:meshHeading |
pubmed-meshheading:20383584-Animals,
pubmed-meshheading:20383584-Apoptosis,
pubmed-meshheading:20383584-Caspase 3,
pubmed-meshheading:20383584-Cell Hypoxia,
pubmed-meshheading:20383584-Chemokine CXCL12,
pubmed-meshheading:20383584-Culture Media, Serum-Free,
pubmed-meshheading:20383584-Cytoprotection,
pubmed-meshheading:20383584-Enzyme Activation,
pubmed-meshheading:20383584-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:20383584-Flow Cytometry,
pubmed-meshheading:20383584-Humans,
pubmed-meshheading:20383584-MAP Kinase Signaling System,
pubmed-meshheading:20383584-Male,
pubmed-meshheading:20383584-Membrane Potential, Mitochondrial,
pubmed-meshheading:20383584-Mesenchymal Stem Cells,
pubmed-meshheading:20383584-Mitochondria,
pubmed-meshheading:20383584-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:20383584-Phosphorylation,
pubmed-meshheading:20383584-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:20383584-Rats,
pubmed-meshheading:20383584-Rats, Sprague-Dawley
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
SDF-1? inhibits hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells through PI3K/Akt and ERK1/2 signaling pathways.
|
| pubmed:affiliation |
Research Center for Cardiac Regenerative Medicine, The Ministry of Health, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|