Linked Life Data

20331619

Source:http://linkedlifedata.com/resource/pubmed/id/20331619

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-8-10
pubmed:abstractText
The newly proposed glioma stem cell (GSC) hypothesis may re-model the way we diagnose and treat the tumor, which highlights the need for a complete knowledge on the genetic and epigenetic "blueprints" of GSCs. To identify the true "stemness" signatures, pure GSC populations are primarily needed. Reliable in vitro methods enriching for GSCs and thereby identifying the key stem-like characteristics constitute the preliminary step forward. We discuss in this review the current widely used methods for enriching and isolating GSCs, namely neurosphere assay, CD133 Immunophenotyping and side population assay, and detail their limitations and potential pitfalls that could complicate interpretation of corresponding results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1750-3639
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
877-89
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
The utility and limitations of neurosphere assay, CD133 immunophenotyping and side population assay in glioma stem cell research.
pubmed:affiliation
Department of Neurosurgery, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China. wanruiyan@hotmail.com
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't