Linked Life Data

20307542

Source:http://linkedlifedata.com/resource/pubmed/id/20307542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-5-10
pubmed:abstractText
In vitro stimulation with fresh and frozen peripheral blood mononuclear cells (PBMCs) or fresh whole blood has been widely used in animal studies and clinical trials to study the immunological features of a number of human diseases. The objective of this study was to determine the difference in response to stimulation of fresh PBMCs, frozen PBMCs, and fresh whole blood by change of TNFalpha gene expression levels after 4-hour in vitro lipopolysaccharide (LPS) stimulation. Our results demonstrate that TNFalpha gene expression significantly increases in both fresh PBMCs and fresh whole blood when 1 microg/ml or 10 microg/ml LPS was used but only after stimulation with 10 microg/ml LPS in frozen PBMCs. Mean fold change of TNFalpha gene expression levels after 1 microg/ml LPS stimulation was significantly higher using fresh whole blood (51.01+/-7.91) as compared to fresh PBMCs (8.92+/-2.16) or frozen PBMCs (3.04+/-0.55) (p<or=0.007). The same trend was also observed with 10 microg/ml LPS stimulation (p<or=0.001). Decreased PBMC cell viability due to cryopreservation, cytokine depletion and removal of some minor cells during cell isolation could attribute to the low TNFalpha expression in the frozen PBMCs. Our results strongly suggest that fresh whole blood is the best choice for in vitro stimulation studies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1872-7905
pubmed:author
pubmed:issnType
Electronic
pubmed:day
31
pubmed:volume
357
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-7
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Comparative in vitro stimulation with lipopolysaccharide to study TNFalpha gene expression in fresh whole blood, fresh and frozen peripheral blood mononuclear cells.
pubmed:affiliation
Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. jiwang@northwestern.edu <jiwang@northwestern.edu>
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't