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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2010-4-1
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pubmed:abstractText |
The size and integrity of the Golgi apparatus is maintained via a tightly controlled regulation of membrane traffic using a variety of different signaling and cytoskeletal proteins. We have recently observed that activation of c-Src has profound effects on Golgi structure, leading to dramatically vesiculated cisternae in a variety of cell types. As the large GTPase dynamin (Dyn2) has been implicated in Golgi vesiculation during secretion, we tested whether inhibiting Dyn2 activity by expression of a Dyn2K44A mutant or siRNA knockdown could attenuate active Src-induced Golgi fragmentation. Indeed, these perturbations attenuated fragmentation, and expression of a Dyn2Y(231/597)F mutant protein that cannot be phosphorylated by Src kinase had a similar effect . Finally, we find that Dyn2 is markedly phosphorylated during the transit of VSV-G protein through the TGN whereas expression of the Dyn2Y(231/597)F mutant significantly reduces exit of the nascent protein from this compartment. These findings demonstrate that activation of Dyn2 by Src kinase regulates Golgi integrity and vesiculation during the secretory process.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
30
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pubmed:volume |
107
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5863-8
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pubmed:dateRevised |
2011-3-14
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pubmed:meshHeading |
pubmed-meshheading:20231454-Amino Acid Substitution,
pubmed-meshheading:20231454-Animals,
pubmed-meshheading:20231454-Cell Line, Tumor,
pubmed-meshheading:20231454-Cells, Cultured,
pubmed-meshheading:20231454-Cricetinae,
pubmed-meshheading:20231454-Dynamin II,
pubmed-meshheading:20231454-Golgi Apparatus,
pubmed-meshheading:20231454-HeLa Cells,
pubmed-meshheading:20231454-Humans,
pubmed-meshheading:20231454-Membrane Glycoproteins,
pubmed-meshheading:20231454-Mutagenesis, Site-Directed,
pubmed-meshheading:20231454-Pancreatic Neoplasms,
pubmed-meshheading:20231454-Phenotype,
pubmed-meshheading:20231454-Phosphorylation,
pubmed-meshheading:20231454-RNA, Small Interfering,
pubmed-meshheading:20231454-Rats,
pubmed-meshheading:20231454-Recombinant Proteins,
pubmed-meshheading:20231454-Transfection,
pubmed-meshheading:20231454-Viral Envelope Proteins,
pubmed-meshheading:20231454-src-Family Kinases,
pubmed-meshheading:20231454-trans-Golgi Network
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pubmed:year |
2010
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pubmed:articleTitle |
Src kinase regulates the integrity and function of the Golgi apparatus via activation of dynamin 2.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology and the Miles and Shirley Fiterman Center for Digestive Diseases, Mayo Clinic, Rochester, MN, 55905, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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