Source:http://linkedlifedata.com/resource/pubmed/id/20228168
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2010-4-22
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| pubmed:abstractText |
Previously, we showed that levels of different CCAAT/enhancer binding protein (C/EBP) mRNAs in the liver of rainbow trout were modulated by GH and suggested that C/EBPs might be involved in GH-induced IGF-II gene expression. As a step toward further investigation, we have developed monospecific polyclonal antibodies to detect rainbow trout C/EBPalpha, -beta1, -beta2, and -delta2 isoform proteins. Injection of GH into adult rainbow trout resulted in a significant increase of C/EBPbeta1, C/EBPbeta2, and C/EBPdelta2 proteins in the liver. Chromatin immunoprecipitation analysis revealed that C/EBPbeta2 binds to multiple sites at the 5' promoter/regulatory region, introns, and the 3' untranslated region of the IGF-II gene. GH treatment reduced C/EBPbeta2 binding to several of these regions at 6 h after injection. The decreased occupancy of C/EBPbeta2 coincided well with an increase of histone H4 acetylation at the proximal promoter and elevation of the IGF-II mRNA level. Immunoblotting analysis showed that C/EBPbeta2 existed predominately as a truncated form in the liver, and cotransfection analysis further showed that the truncated C/EBPbeta2 acted as a negative regulator on IGF-II proximal promoter. GH treatment caused deacetylation of C/EBPbeta2 in the liver. In addition, we observed a GH-dependent interaction of C/EBPbeta2 with a complex involving histone H1. All together, these results suggest that C/EBPbeta2 was regulated at multiple levels by GH, and C/EBPbeta2 may play a suppressive role in mediating GH-induced IGF-II expression in the liver of rainbow trout.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Fish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor II
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1945-7170
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
151
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2128-39
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| pubmed:meshHeading |
pubmed-meshheading:20228168-Acetylation,
pubmed-meshheading:20228168-Animals,
pubmed-meshheading:20228168-Binding Sites,
pubmed-meshheading:20228168-CCAAT-Enhancer-Binding Protein-beta,
pubmed-meshheading:20228168-Cell Line, Tumor,
pubmed-meshheading:20228168-Chromatin Immunoprecipitation,
pubmed-meshheading:20228168-Fish Proteins,
pubmed-meshheading:20228168-Gene Expression Regulation,
pubmed-meshheading:20228168-Growth Hormone,
pubmed-meshheading:20228168-Histones,
pubmed-meshheading:20228168-Immunoblotting,
pubmed-meshheading:20228168-Insulin-Like Growth Factor II,
pubmed-meshheading:20228168-Liver,
pubmed-meshheading:20228168-Oncorhynchus mykiss,
pubmed-meshheading:20228168-Promoter Regions, Genetic,
pubmed-meshheading:20228168-Protein Binding,
pubmed-meshheading:20228168-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20228168-Transfection
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| pubmed:year |
2010
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| pubmed:articleTitle |
CCAAT/enhancer binding protein beta2 is involved in growth hormone-regulated insulin-like growth factor-II gene expression in the liver of rainbow trout (Oncorhynchus mykiss).
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| pubmed:affiliation |
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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