Linked Life Data

20210479

Source:http://linkedlifedata.com/resource/pubmed/id/20210479

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-3-9
pubmed:abstractText
A simple optical method using hollow-core photonic crystal fiber for protein detection has been described. In this study, estrogen receptor (ER) from a MCF-7 breast carcinoma cell lysates immobilized inside a hollow-core photonic crystal fiber was detected using anti-ER primary antibody with either Alexa Fluor 488 (green fluorescent dye) or 555 (red Fluorescent dye) labeled Goat anti-rabbit IgG as the secondary antibody. The fluorescence fingerprints of the ERalpha protein were observed under fluorescence microscope, and its optical characteristics were analyzed. The ERalpha protein detection by this proposed method is based on immuno binding from sample volume as low as 50 nL. This method is expected to offer great potential as a biosensor for medical diagnostics and therapeutics applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1560-2281
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
017005
pubmed:meshHeading
pubmed:articleTitle
Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume.
pubmed:affiliation
Nanyang Technological University, School of Mechanical and Aerospace Engineering, Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't