Linked Life Data

20204851

Source:http://linkedlifedata.com/resource/pubmed/id/20204851

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-3-5
pubmed:abstractText
Utilization of structural information from homologous proteins to design novel enzymes is one of the practical applications of structural biology. Structure-based protein engineering is a more reasonable strategy compared with general random mutagenesis. Here, we describe a useful method for production of a series of mutant enzymes based on a cell-free translation system. We employed PCR-mediated in vitro site-directed mutagenesis in combination with wheat-embryo cell-free protein synthesis to establish a high-throughput system. The efficient generation of a series of mutant enzymes facilitates high-throughput screening of functionally improved enzymes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
607
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
85-99
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Protein engineering accelerated by cell-free technology.
pubmed:affiliation
Cell-Free Science and Technology Research Center and Venture Business Laboratory, Ehime University, Ehime, Japan.
pubmed:publicationType
Journal Article