Linked Life Data

20199089

Source:http://linkedlifedata.com/resource/pubmed/id/20199089

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-3-18
pubmed:abstractText
Fluorescence recovery after photobleaching (FRAP) is one of the most powerful and used techniques to study diffusion processes of macromolecules in membranes or in bulk. Here, we study the diffusion of alpha-chymotrypsin in different crowded (Dextran) in vitro solutions using a confocal laser scanning microscope. In the considered experimental conditions, confocal FRAP images could be analyzed applying the uniform circular disk approximation described for a nonscanning microscope generalized to take into account anomalous diffusion. Considering the slow diffusion of macromolecules in crowded media, we compare the fitting of confocal FRAP curves analyzed with the equations provided by the Gaussian and the uniform circular disk profile models for nonscanning microscopes. As the fitted parameter variation with the size and concentration of crowders is qualitatively similar for both models, the use of the uniform circular disk or the Gaussian model is justified for these experiments. Moreover, in our experimental conditions, alpha-chymotrypsin shows anomalous diffusion (alpha < 1), depending on the size and concentration of Dextran molecules, until a high concentration and high size of crowding agent are achieved. This result indicates a range of validity of the idealized fitting expressions used, beyond which other physical phenomena must be considered.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1520-5207
pubmed:author
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4028-34
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Diffusion of alpha-chymotrypsin in solution-crowded media. A fluorescence recovery after photobleaching study.
pubmed:affiliation
Department of Physical Chemistry and Research Institute of Theoretical and Computational Chemistry of University of Barcelona, Barcelona, Spain. i.pastor@ub.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't