Linked Life Data

20181891

Source:http://linkedlifedata.com/resource/pubmed/id/20181891

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-3-22
pubmed:databankReference
pubmed:abstractText
Emerging data suggest that regulatory T cell (Treg) dysfunction and consequent breakdown of immunological self-tolerance in autoimmunity can be mediated by factors that are not Treg-intrinsic (e.g., cytokines). Indeed, recent studies show that in rheumatoid arthritis the proinflammatory cytokine TNF reduces the suppressive function of Tregs, whereas in vivo TNF blockade restores this function and accordingly self-tolerance. However, until now a coherent mechanism by which TNF regulates the Treg has not been described. In this paper, we show that TNF induces preferential and significant activation of the canonical NF-kappaB pathway in human Tregs as compared with CD25(-) conventional T cells. Furthermore, TNF induced primarily in CD45RA(-) Tregs a transcription program highly enriched for typical NF-kappaB target genes, such as the cytokines lymphotoxin-alpha and TNF, the TNFR superfamily members FAS, 4-1BB, and OX-40, various antiapoptotic genes, and other important immune-response genes. FACS analysis revealed that TNF also induced upregulation of cell surface expression of 4-1BB and OX40 specifically in CD45RA(-)FOXP3(+) Tregs. In contrast, TNF had only a minimal effect on the Treg's core transcriptional signature or on the intracellular levels of the FOXP3 protein in Tregs. Importantly, TNF treatment modulated the capacity of Tregs to suppress the proliferation and IFN-gamma secretion by conventional T cells, an effect that was fully reversed by cotreatment with anti-TNFR2 mAbs. Our findings thus provide new mechanistic insight into the role of TNF and TNFR2 in the pathogenesis of autoimmunity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1550-6606
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
184
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3570-81
pubmed:meshHeading
pubmed-meshheading:20181891-Antigens, CD45, pubmed-meshheading:20181891-Autoimmunity, pubmed-meshheading:20181891-Cell Separation, pubmed-meshheading:20181891-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:20181891-Flow Cytometry, pubmed-meshheading:20181891-Forkhead Transcription Factors, pubmed-meshheading:20181891-Gene Expression, pubmed-meshheading:20181891-Gene Expression Regulation, pubmed-meshheading:20181891-Humans, pubmed-meshheading:20181891-Immune Tolerance, pubmed-meshheading:20181891-Interferon-gamma, pubmed-meshheading:20181891-Lymphocyte Activation, pubmed-meshheading:20181891-NF-kappa B, pubmed-meshheading:20181891-Receptors, Tumor Necrosis Factor, Type II, pubmed-meshheading:20181891-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20181891-Signal Transduction, pubmed-meshheading:20181891-T-Lymphocyte Subsets, pubmed-meshheading:20181891-T-Lymphocytes, Regulatory, pubmed-meshheading:20181891-Tumor Necrosis Factor-alpha
pubmed:year
2010
pubmed:articleTitle
TNF activates a NF-kappaB-regulated cellular program in human CD45RA- regulatory T cells that modulates their suppressive function.
pubmed:affiliation
Sheba Cancer Research Center, Tel Aviv University, Sackler Faculty of Medicine, Tel Hashomer, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't