Linked Life Data

20087163

Source:http://linkedlifedata.com/resource/pubmed/id/20087163

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-2-11
pubmed:abstractText
Chronic kidney disease affects 1 of 9 Americans. Recent studies showed increased activation of the metalloenzyme disintegrin ADAM-17 during the development of the disease and that threonine phosphorylation of ADAM-17 may be an important regulator of the enzyme activity. Using kidney mesangial cells we investigated whether profibrotic serotonin (5-HT) induces phosphorylation of ADAM-17 with concomitant increase in the enzyme activity. We found that 5-HT treatment (1 mM for 10 minutes) induced a significant 3-fold increase in ADAM-17 phosphorylation and employing a fluorogenic enzyme activity assay we showed 2.3-fold activation of ADAM-17, both of which was inhibited by PD98059 (1 mM), an inhibitor of extracellular signal regulated kinase (ERK) activation. In coimmunoprecipitation analysis, we observed increased (2.7-fold) binding of activated ERK to ADAM-17 during 5-HT stimulation. We concluded that, during profibrotic stimulus, ERK phosphorylates ADAM-17 in kidney cells which induces concomitant increase in the enzyme activity.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1538-2990
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
339
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
105-7
pubmed:dateRevised
2011-7-22
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
ADAM-17 is activated by the mitogenic protein kinase ERK in a model of kidney fibrosis.
pubmed:affiliation
Medical University of South Carolina, Charleston, 29425, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural