Linked Life Data

20057048

Source:http://linkedlifedata.com/resource/pubmed/id/20057048

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
2010-1-8
pubmed:abstractText
The second virial coefficient, or B value, is a measurement of how well a protein interacts with itself in solution. These interactions can lead to protein crystallization or precipitation, depending on their strength, with a narrow range of B values (the 'crystallization slot') being known to promote crystallization. A convenient method of determining the B value is by self-interaction chromatography. This paper describes how the light-harvesting complex 1-reaction centre core complex from Allochromatium vinosum yielded single straight-edged crystals after iterative cycles of self-interaction chromatography and crystallization. This process allowed the rapid screening of small molecules and detergents as crystallization additives. Here, a description is given of how self-interaction chromatography has been utilized to improve the crystallization conditions of a membrane protein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1399-0047
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
44-50
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Self-interaction chromatography as a tool for optimizing conditions for membrane protein crystallization.
pubmed:affiliation
Division of Molecular and Cellular Biology, Faculty of Biological Life Sciences, University of Glasgow, Scotland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't