Source:http://linkedlifedata.com/resource/pubmed/id/19966073
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
2010-2-1
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| pubmed:abstractText |
The MRS2/MGT gene family in Arabidopsis thaliana belongs to the superfamily of CorA-MRS2-ALR-type membrane proteins. Proteins of this type are characterized by a GMN tripeptide motif (Gly-Met-Asn) at the end of the first of two C-terminal transmembrane domains and have been characterized as magnesium transporters. Using the recently established mag-fura-2 system allowing direct measurement of Mg(2+) uptake into mitochondria of Saccharomyces cerevisiae, we find that all members of the Arabidopsis family complement the corresponding yeast mrs2 mutant. Highly different patterns of tissue-specific expression were observed for the MRS2/MGT family members in planta. Six of them are expressed in root tissues, indicating a possible involvement in plant magnesium supply and distribution after uptake from the soil substrate. Homozygous T-DNA insertion knockout lines were obtained for four members of the MRS2/MGT gene family. A strong, magnesium-dependent phenotype of growth retardation was found for mrs2-7 when Mg(2+) concentrations were lowered to 50 microM in hydroponic cultures. Ectopic overexpression of MRS2-7 from the cauliflower mosaic virus 35S promoter results in complementation and increased biomass accumulation. Green fluorescent protein reporter gene fusions indicate a location of MRS2-7 in the endomembrane system. Hence, contrary to what is frequently found in analyses of plant gene families, a single gene family member knockout results in a strong, environmentally dependent phenotype.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cation Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/T-DNA
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
|
| pubmed:issn |
1532-298X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4018-30
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| pubmed:dateRevised |
2011-3-3
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| pubmed:meshHeading |
pubmed-meshheading:19966073-Arabidopsis,
pubmed-meshheading:19966073-Arabidopsis Proteins,
pubmed-meshheading:19966073-Cation Transport Proteins,
pubmed-meshheading:19966073-Cloning, Molecular,
pubmed-meshheading:19966073-DNA, Bacterial,
pubmed-meshheading:19966073-Gene Expression Regulation, Plant,
pubmed-meshheading:19966073-Gene Knockout Techniques,
pubmed-meshheading:19966073-Genetic Complementation Test,
pubmed-meshheading:19966073-Magnesium,
pubmed-meshheading:19966073-Multigene Family,
pubmed-meshheading:19966073-Mutagenesis, Insertional,
pubmed-meshheading:19966073-Mutation,
pubmed-meshheading:19966073-Phylogeny,
pubmed-meshheading:19966073-Plant Roots,
pubmed-meshheading:19966073-Plants, Genetically Modified,
pubmed-meshheading:19966073-RNA, Plant,
pubmed-meshheading:19966073-Saccharomyces cerevisiae,
pubmed-meshheading:19966073-Tobacco
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| pubmed:year |
2009
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| pubmed:articleTitle |
A root-expressed magnesium transporter of the MRS2/MGT gene family in Arabidopsis thaliana allows for growth in low-Mg2+ environments.
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| pubmed:affiliation |
Institut für Zelluläre und Molekulare Botanik, Universität Bon, D-53115 Bon, Germany.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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