| pubmed-article:19954742 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C0006142 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C0812281 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C0596890 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C0521451 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C0010656 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:19954742 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:19954742 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:19954742 | pubmed:dateCreated | 2010-1-27 | lld:pubmed |
| pubmed-article:19954742 | pubmed:abstractText | Surfactin has been known to inhibit proliferation and induce apoptosis in cancer cells. However, the molecular mechanisms involved in surfactin-induced apoptosis remain poorly understood. The present study was undertaken to elucidate the underlying network of signaling events in surfactin-induced apoptosis of human breast cancer MCF-7 cells. In this study, surfactin caused reactive oxygen species (ROS) generation and the surfactin-induced cell death was prevented by antioxidants N-acetylcysteine (NAC) and catalase, suggesting involvement of ROS generation in surfactin-induced cell death. Surfactin induced a sustained activation of the phosphorylation of ERK1/2 and JNK, but not p38. Moreover, surfactin-induced cell death was reversed by PD98059 (an inhibitor of ERK1/2) and SP600125 (an inhibitor of JNK), but not by SB203580 (an inhibitor of p38). However, the phosphorylation of JNK rather than ERK1/2 activation by surfactin was blocked by NAC/catalase. These results suggest that the action of surfactin on MCF-7 cells was via ERK1/2 and JNK, but not via p38, and the ERK1/2 and JNK activation induce apoptosis through two independent signaling mechanisms. Surfactin triggered the mitochondrial/caspase apoptotic pathway indicated by enhanced Bax-to-Bcl-2 expression ratio, loss of mitochondrial membrane potential, cytochrome c release, and caspase cascade reaction. The NAC and SP600125 blocked these events induced by surfactin. Moreover, the general caspase inhibitor z-VAD-FMK inhibited the caspase-6 activity and exerted the protective effect against the surfactin-induced cell death. Taken together, these findings suggest that the surfactin induces apoptosis through a ROS/JNK-mediated mitochondrial/caspase pathway. | lld:pubmed |
| pubmed-article:19954742 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19954742 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19954742 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19954742 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19954742 | pubmed:issn | 1872-7786 | lld:pubmed |
| pubmed-article:19954742 | pubmed:author | pubmed-author:WangAi-huaAH | lld:pubmed |
| pubmed-article:19954742 | pubmed:author | pubmed-author:WangChun-ling... | lld:pubmed |
| pubmed-article:19954742 | pubmed:author | pubmed-author:CaoXiao-hongX... | lld:pubmed |
| pubmed-article:19954742 | pubmed:author | pubmed-author:MaoDe-zhiDZ | lld:pubmed |
| pubmed-article:19954742 | pubmed:author | pubmed-author:LuMei-fangMF | lld:pubmed |
| pubmed-article:19954742 | pubmed:author | pubmed-author:CuiYun-qianYQ | lld:pubmed |
| pubmed-article:19954742 | pubmed:author | pubmed-author:JiaoRun-zhiRZ | lld:pubmed |
| pubmed-article:19954742 | pubmed:copyrightInfo | Copyright (c) 2009 Elsevier Ireland Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:19954742 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19954742 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:19954742 | pubmed:volume | 183 | lld:pubmed |
| pubmed-article:19954742 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19954742 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19954742 | pubmed:pagination | 357-62 | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:meshHeading | pubmed-meshheading:19954742... | lld:pubmed |
| pubmed-article:19954742 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:19954742 | pubmed:articleTitle | Surfactin induces apoptosis in human breast cancer MCF-7 cells through a ROS/JNK-mediated mitochondrial/caspase pathway. | lld:pubmed |
| pubmed-article:19954742 | pubmed:affiliation | Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China. wahwawayu@126.com | lld:pubmed |
| pubmed-article:19954742 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19954742 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19954742 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19954742 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19954742 | lld:pubmed |
