Source:http://linkedlifedata.com/resource/pubmed/id/19954742
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2010-1-27
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| pubmed:abstractText |
Surfactin has been known to inhibit proliferation and induce apoptosis in cancer cells. However, the molecular mechanisms involved in surfactin-induced apoptosis remain poorly understood. The present study was undertaken to elucidate the underlying network of signaling events in surfactin-induced apoptosis of human breast cancer MCF-7 cells. In this study, surfactin caused reactive oxygen species (ROS) generation and the surfactin-induced cell death was prevented by antioxidants N-acetylcysteine (NAC) and catalase, suggesting involvement of ROS generation in surfactin-induced cell death. Surfactin induced a sustained activation of the phosphorylation of ERK1/2 and JNK, but not p38. Moreover, surfactin-induced cell death was reversed by PD98059 (an inhibitor of ERK1/2) and SP600125 (an inhibitor of JNK), but not by SB203580 (an inhibitor of p38). However, the phosphorylation of JNK rather than ERK1/2 activation by surfactin was blocked by NAC/catalase. These results suggest that the action of surfactin on MCF-7 cells was via ERK1/2 and JNK, but not via p38, and the ERK1/2 and JNK activation induce apoptosis through two independent signaling mechanisms. Surfactin triggered the mitochondrial/caspase apoptotic pathway indicated by enhanced Bax-to-Bcl-2 expression ratio, loss of mitochondrial membrane potential, cytochrome c release, and caspase cascade reaction. The NAC and SP600125 blocked these events induced by surfactin. Moreover, the general caspase inhibitor z-VAD-FMK inhibited the caspase-6 activity and exerted the protective effect against the surfactin-induced cell death. Taken together, these findings suggest that the surfactin induces apoptosis through a ROS/JNK-mediated mitochondrial/caspase pathway.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 6,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides, Cyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/surfactin peptide
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1872-7786
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright (c) 2009 Elsevier Ireland Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
12
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| pubmed:volume |
183
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
357-62
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| pubmed:meshHeading |
pubmed-meshheading:19954742-Antibiotics, Antineoplastic,
pubmed-meshheading:19954742-Apoptosis,
pubmed-meshheading:19954742-Breast Neoplasms,
pubmed-meshheading:19954742-Caspase 6,
pubmed-meshheading:19954742-Cell Line, Tumor,
pubmed-meshheading:19954742-Cytochromes c,
pubmed-meshheading:19954742-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:19954742-Female,
pubmed-meshheading:19954742-Humans,
pubmed-meshheading:19954742-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:19954742-Lipopeptides,
pubmed-meshheading:19954742-MAP Kinase Signaling System,
pubmed-meshheading:19954742-Membrane Potential, Mitochondrial,
pubmed-meshheading:19954742-Mitochondria,
pubmed-meshheading:19954742-Peptides, Cyclic,
pubmed-meshheading:19954742-Phosphorylation,
pubmed-meshheading:19954742-Reactive Oxygen Species,
pubmed-meshheading:19954742-bcl-2-Associated X Protein,
pubmed-meshheading:19954742-bcl-X Protein
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| pubmed:year |
2010
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| pubmed:articleTitle |
Surfactin induces apoptosis in human breast cancer MCF-7 cells through a ROS/JNK-mediated mitochondrial/caspase pathway.
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| pubmed:affiliation |
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China. wahwawayu@126.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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