Linked Life Data

19886251

Source:http://linkedlifedata.com/resource/pubmed/id/19886251

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-11-4
pubmed:abstractText
Equine herpesvirus type 1 (EHV-1) is one of the major viral agents causing diseases in horses common worldwide. A variety of techniques, including PCR, have been used to diagnose EHV-1 infections. In this paper, an attempt of real-time PCR has been described, which uses specific fluorochrome-labeled TaqMan probes for detection of viral DNA. This method does not require post-amplification manipulations, thereby reducing the risk of cross-contamination. The assay was sensitive enough to detect EHV-1 sequences in different clinical samples, as well in mice neuronal cell cultures. The technique was also very specific--here was no cross reaction with other human and equine herpesviruses. Compared to previously used nested PCR technique, the test was more sensitive and should be useful for the common diagnosis based on its specificity and rapidity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1505-1773
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
311-5
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Equine herpesvirus type 1 quantification in different types of samples by a real-time PCR.
pubmed:affiliation
Chair and Department of Medical Microbiology, Faculty of Medicine, Medical University of Warsaw, Cha?ubi?skiego 5, 02-004 Warsaw, Poland. dzieciatkowski@wp.pl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't