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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-2-1
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pubmed:abstractText |
Leukocyte function associated antigen-1 (LFA-1) is a primary cell adhesion molecule of leukocytes required for mediating cellular transmigration into sites of inflammation via the vascular endothelium. A cyclic peptide, cIBR, possesses high affinity for LFA-1, and conjugation to the surface of poly(DL-lactic-co-glycolic acid) nanoparticles can specifically target and deliver the encapsulated agents to T cells expressing LFA-1. The kinetics of targeted nanoparticle uptake by acute lymphoblastic leukemia T cells was investigated by flow cytometry and microscopy and compared to untargeted nanoparticles. The specificity of targeted nanoparticles binding to the LFA-1 integrin was demonstrated by competitive inhibition using free cIBR peptide or using the I domain of LFA-1 to inhibit the binding of targeted nanoparticles. The uptake of targeted nanoparticles was concentration and energy dependent. The cIBR-conjugated nanoparticles did not appear to localize with lysosomes whereas untargeted nanoparticles were detected in lysosomes in 6 h and steadily accumulated in lysosomes for 24 h. Finally, T-cell adhesion to epithelial cells was inhibited by cIBR nanoparticles. Thus, nanoparticles displaying the cIBR ligand may offer a useful targeted drug delivery system as an alternative treatment of inflammatory diseases involving transmigration of leukocytes.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/P20 RR015563-060017,
http://linkedlifedata.com/resource/pubmed/grant/P20 RR015563-076485,
http://linkedlifedata.com/resource/pubmed/grant/P20 RR015563-086731,
http://linkedlifedata.com/resource/pubmed/grant/P20 RR016443,
http://linkedlifedata.com/resource/pubmed/grant/P20 RR016443-086923,
http://linkedlifedata.com/resource/pubmed/grant/P20 RR016443-096682,
http://linkedlifedata.com/resource/pubmed/grant/P20 RR016443-105872,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI063002-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI063002-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI063002-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI063002-04,
http://linkedlifedata.com/resource/pubmed/grant/R03 AR054035,
http://linkedlifedata.com/resource/pubmed/grant/R03 AR054035-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R03 AR054035-02,
http://linkedlifedata.com/resource/pubmed/grant/R03 AR054035-03,
http://linkedlifedata.com/resource/pubmed/grant/T32 GM08359-11
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-10507489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-10553036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-10613446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-10861083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-11035201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-11544294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-11606914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-11857637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-12020219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-12234370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-12636159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-14620502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-14733906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-15319185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-16207819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-16305394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-16923022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-17680719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-19429421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-3517218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-3911593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-7680658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-8658886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-8856611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-8871677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19883077-9278346
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1543-8392
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
146-55
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:19883077-Biological Transport, Active,
pubmed-meshheading:19883077-Cell Adhesion,
pubmed-meshheading:19883077-Cell Aggregation,
pubmed-meshheading:19883077-Cell Line, Tumor,
pubmed-meshheading:19883077-Drug Delivery Systems,
pubmed-meshheading:19883077-Humans,
pubmed-meshheading:19883077-Lactic Acid,
pubmed-meshheading:19883077-Ligands,
pubmed-meshheading:19883077-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:19883077-Lysosomes,
pubmed-meshheading:19883077-Microscopy, Fluorescence,
pubmed-meshheading:19883077-Nanoparticles,
pubmed-meshheading:19883077-Peptides, Cyclic,
pubmed-meshheading:19883077-Polyglycolic Acid,
pubmed-meshheading:19883077-Precursor T-Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:19883077-T-Lymphocytes,
pubmed-meshheading:19883077-Temperature
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pubmed:year |
2010
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pubmed:articleTitle |
cIBR effectively targets nanoparticles to LFA-1 on acute lymphoblastic T cells.
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pubmed:affiliation |
Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, Kansas 66047, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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