Source:http://linkedlifedata.com/resource/pubmed/id/19875126
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
19
|
| pubmed:dateCreated |
2010-4-19
|
| pubmed:abstractText |
Tumor related products shed into the feces offer a potential source of biomarkers for the detection of colorectal cancer (CRC). Using SDS-PAGE followed by nanoflow reversed-phased LC-MS/MS to analyse fecal samples from Apc(Min/+) mice (that develop spontaneous multiple intestinal neoplasia with age) we have identified 336 proteins (115 proteins of murine origin, 201 from fecal bacteria, 18 associated with food intake and 2 of apparent parasitic origin). 75% of the murine proteins identified in this study are predicted to be extracellular or associated with the cell plasma membrane. Of these proteins, a number of the murine homologues of colorectal cancer associated proteins (CCAP) such as hemoglobin, haptoglobin, hemopexin, alpha-2-macroglobulin and cadherin-17 have been identified, demonstrating the potential of fecal proteomics for detecting potential biomarkers and paving the way for subsequent MS/MS based biomarker studies on similar human samples.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1873-3778
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright (c) 2009 Elsevier B.V. All rights reserved.
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
7
|
| pubmed:volume |
1217
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3330-40
|
| pubmed:meshHeading |
pubmed-meshheading:19875126-Age Factors,
pubmed-meshheading:19875126-Animals,
pubmed-meshheading:19875126-Bacterial Proteins,
pubmed-meshheading:19875126-Chromatography, Liquid,
pubmed-meshheading:19875126-Cluster Analysis,
pubmed-meshheading:19875126-Colorectal Neoplasms,
pubmed-meshheading:19875126-Disease Models, Animal,
pubmed-meshheading:19875126-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:19875126-Feces,
pubmed-meshheading:19875126-Genes, APC,
pubmed-meshheading:19875126-Mice,
pubmed-meshheading:19875126-Mice, Inbred C57BL,
pubmed-meshheading:19875126-Mice, Transgenic,
pubmed-meshheading:19875126-Neoplasm Proteins,
pubmed-meshheading:19875126-Proteomics,
pubmed-meshheading:19875126-Sequence Homology, Amino Acid,
pubmed-meshheading:19875126-Subcellular Fractions,
pubmed-meshheading:19875126-Tandem Mass Spectrometry,
pubmed-meshheading:19875126-Tumor Markers, Biological
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Murine fecal proteomics: a model system for the detection of potential biomarkers for colorectal cancer.
|
| pubmed:affiliation |
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, PO Box 2008, Royal Melbourne Hospital, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|