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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-2-19
|
| pubmed:abstractText |
Hamsters were superovulated and their oocytes frozen in Dulbecco's phosphate-buffered saline containing 10% fetal calf serum and 1.5 molar 1,2-propanediol. In several experiments, 94% of the hamster oocytes survived freezing and were equivalent to fresh oocytes in sperm penetration. In routine use of the procedure described, also 94% of 1,340 frozen-thawed oocytes were satisfactory for sperm penetration of several species. Frozen oocytes eliminate problems and costs of maintaining hamster colonies with variable responses on individual days.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0015-0282
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
55
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
184-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1986958-Analysis of Variance,
pubmed-meshheading:1986958-Animals,
pubmed-meshheading:1986958-Cricetinae,
pubmed-meshheading:1986958-Cryopreservation,
pubmed-meshheading:1986958-Female,
pubmed-meshheading:1986958-Fertilization in Vitro,
pubmed-meshheading:1986958-Gonadotropins, Equine,
pubmed-meshheading:1986958-Male,
pubmed-meshheading:1986958-Oocytes,
pubmed-meshheading:1986958-Sperm-Ovum Interactions,
pubmed-meshheading:1986958-Superovulation
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
A procedure for cryopreservation of hamster oocytes yielding highly conserved oocytes suitable for sperm penetration tests.
|
| pubmed:affiliation |
Department of Animal Science, Cornell University Ithaca, New York 14853-4801.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|