Source:http://linkedlifedata.com/resource/pubmed/id/19828811
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
41
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| pubmed:dateCreated |
2009-10-15
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| pubmed:abstractText |
Systemic injection of lipopolysaccharide (LPS) is a widely used model of immune/inflammatory challenge, which can invoke a host of CNS responses, including activation of the hypothalamic-pituitary-adrenal (HPA) axis. Inducible vascular prostaglandin E(2) (PGE(2)) synthesis by endothelial (ECs) and/or perivascular cells (PVCs) (a macrophage-derived vascular cell type) is implicated in the engagement of HPA and other CNS responses, by virtue of their capacity to express cyclooxygenase-2 (COX-2) and microsomal PGE(2) synthase-1. Evidence from genetic and pharmacologic studies also supports a role for the constitutively expressed COX-1 in inflammation-induced activation of the HPA axis, although histochemical evidence to support relevant localization(s) and regulation of COX-1 expression is lacking. The present experiments fill this void in showing that COX-1 immunoreactivity (IR) and mRNA are detectable in identified PVCs and parenchymal microglia under basal conditions and is robustly expressed in these and ECs 1-3 h after intravenous injection of LPS (2 microg/kg). Confocal and electron microscopic analyses indicate distinct cellular/subcellular localizations of COX-1-IR in the three cell types. Interestingly, COX-1 expression is enhanced in ECs of brain PVC-depleted rats, supporting an anti-inflammatory role of the latter cell type. Functional involvement of COX-1 is indicated by the observation that central, but not systemic, pretreatment with the selective COX-1 inhibitor SC-560 attenuated the early phase of LPS-induced increases in adrenocorticotropin and corticosterone secretion. These findings support an involvement of COX-1 in bidirectional interplay between ECs and PVCs in initiating vascular PGE(2) and downstream HPA response to proinflammatory challenges.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenocorticotropic Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Corticosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SC 560,
http://linkedlifedata.com/resource/pubmed/chemical/von Willebrand Factor
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1529-2401
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
14
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| pubmed:volume |
29
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
12970-81
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| pubmed:dateRevised |
2011-5-4
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| pubmed:meshHeading |
pubmed-meshheading:19828811-Adrenocorticotropic Hormone,
pubmed-meshheading:19828811-Animals,
pubmed-meshheading:19828811-Corticosterone,
pubmed-meshheading:19828811-Cyclooxygenase 1,
pubmed-meshheading:19828811-Cyclooxygenase Inhibitors,
pubmed-meshheading:19828811-Dinoprostone,
pubmed-meshheading:19828811-Disease Models, Animal,
pubmed-meshheading:19828811-Gene Expression Regulation,
pubmed-meshheading:19828811-Hypothalamo-Hypophyseal System,
pubmed-meshheading:19828811-Immunoenzyme Techniques,
pubmed-meshheading:19828811-Inflammation,
pubmed-meshheading:19828811-Injections, Intraventricular,
pubmed-meshheading:19828811-Interleukin-1beta,
pubmed-meshheading:19828811-Lipopolysaccharides,
pubmed-meshheading:19828811-Liposomes,
pubmed-meshheading:19828811-Male,
pubmed-meshheading:19828811-Mice,
pubmed-meshheading:19828811-Mice, Knockout,
pubmed-meshheading:19828811-Microscopy, Electron, Transmission,
pubmed-meshheading:19828811-Pituitary-Adrenal System,
pubmed-meshheading:19828811-Pyrazoles,
pubmed-meshheading:19828811-RNA, Messenger,
pubmed-meshheading:19828811-Rats,
pubmed-meshheading:19828811-Rats, Sprague-Dawley,
pubmed-meshheading:19828811-von Willebrand Factor
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| pubmed:year |
2009
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| pubmed:articleTitle |
Cerebrovascular cyclooxygenase-1 expression, regulation, and role in hypothalamic-pituitary-adrenal axis activation by inflammatory stimuli.
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| pubmed:affiliation |
Laboratory of Neuronal Structure and Function, The Salk Institute for Biological Studies and Clayton Medical Research Foundation, La Jolla, California 92037, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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