Linked Life Data

19821945

Source:http://linkedlifedata.com/resource/pubmed/id/19821945

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2011-4-4
pubmed:abstractText
This study describes a method for quantification of transcripts from low numbers of bovine oocytes using real time RT-PCR. The objective was to evaluate the expression pattern of apoptotic genes (Fas, FasL, Bax and Bcl-2) in vitrified-thawed oocytes. Oocytes were evaluated at germinal vesicle stage; at 15 h of maturation; after vitrification and warming at 15 h of maturation and at 9 h of additional maturation. All transcripts showed an increase in at least 1.2-fold change post-vitrification warming, but the levels tended to decrease at 9 h of maturation post-vitrification warming. Transcript abundance for Fas mRNA was 1.4-fold for oocytes after vitrification and warming. The level of Fas mRNA upon maturation was 0.8-fold. The increase in the abundance of FasL mRNA was 2.1, while it was 0.5-fold relative to control. Vitrification resulted in 1.5-fold change in Bax mRNA expression in oocytes. After 9 h of maturation post-vitrification warming, the level for Bax mRNA was 0.6-fold. The mRNA for Bcl-2 was nearly the same after vitrification and warming. The abundance of mRNA for Bcl-2 was 1.2-fold in vitrified oocytes and fell (p = 0.05) to 0.5 at 9 h of maturation post-vitrification and warming. The up-regulation of apoptotic genes in vitrified oocytes may be an early indicator of reduced developmental competence following vitrification. Yet, results from terminal deoxynucleotidyl transferase dUTP nick end labelling and caspase assays did not support the evidence of apoptosis in embryos derived from large numbers of vitrified oocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1439-0531
pubmed:author
pubmed:copyrightInfo
© 2009 Blackwell Verlag GmbH.
pubmed:issnType
Electronic
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
e83-90
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Expression pattern of apoptotic genes in vitrified-thawed bovine oocytes.
pubmed:affiliation
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
pubmed:publicationType
Journal Article