Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7268
pubmed:dateCreated
2009-10-29
pubmed:databankReference
pubmed:abstractText
PIWI-interacting RNAs (piRNAs) silence retrotransposons in Drosophila germ lines by associating with the PIWI proteins Argonaute 3 (AGO3), Aubergine (Aub) and Piwi. piRNAs in Drosophila are produced from intergenic repetitive genes and piRNA clusters by two systems: the primary processing pathway and the amplification loop. The amplification loop occurs in a Dicer-independent, PIWI-Slicer-dependent manner. However, primary piRNA processing remains elusive. Here we analysed piRNA processing in a Drosophila ovarian somatic cell line where Piwi, but not Aub or AGO3, is expressed; thus, only the primary piRNAs exist. In addition to flamenco, a Piwi-specific piRNA cluster, traffic jam (tj), a large Maf gene, was determined as a new piRNA cluster. piRNAs arising from tj correspond to the untranslated regions of tj messenger RNA and are sense-oriented. piRNA loading on to Piwi may occur in the cytoplasm. zucchini, a gene encoding a putative cytoplasmic nuclease, is required for tj-derived piRNA production. In tj and piwi mutant ovaries, somatic cells fail to intermingle with germ cells and Fasciclin III is overexpressed. Loss of tj abolishes Piwi expression in gonadal somatic cells. Thus, in gonadal somatic cells, tj gives rise simultaneously to two different molecules: the TJ protein, which activates Piwi expression, and piRNAs, which define the Piwi targets for silencing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Argonaute Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, Neuronal, http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases, http://linkedlifedata.com/resource/pubmed/chemical/Fas3 (fasciclin 3) protein..., http://linkedlifedata.com/resource/pubmed/chemical/Maf Transcription Factors, Large, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, http://linkedlifedata.com/resource/pubmed/chemical/RNA-Induced Silencing Complex, http://linkedlifedata.com/resource/pubmed/chemical/piwi protein, Drosophila, http://linkedlifedata.com/resource/pubmed/chemical/traffic jam protein, Drosophila, http://linkedlifedata.com/resource/pubmed/chemical/zucchini protein, Drosophila
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1476-4687
pubmed:author
pubmed:issnType
Electronic
pubmed:day
29
pubmed:volume
461
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1296-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19812547-Animals, pubmed-meshheading:19812547-Argonaute Proteins, pubmed-meshheading:19812547-Cell Adhesion Molecules, Neuronal, pubmed-meshheading:19812547-Cell Line, pubmed-meshheading:19812547-Drosophila Proteins, pubmed-meshheading:19812547-Drosophila melanogaster, pubmed-meshheading:19812547-Endoribonucleases, pubmed-meshheading:19812547-Female, pubmed-meshheading:19812547-Genes, Insect, pubmed-meshheading:19812547-Genetic Loci, pubmed-meshheading:19812547-Maf Transcription Factors, Large, pubmed-meshheading:19812547-Male, pubmed-meshheading:19812547-Ovary, pubmed-meshheading:19812547-Phenotype, pubmed-meshheading:19812547-Proto-Oncogene Proteins, pubmed-meshheading:19812547-RNA, pubmed-meshheading:19812547-RNA Interference, pubmed-meshheading:19812547-RNA Processing, Post-Transcriptional, pubmed-meshheading:19812547-RNA-Induced Silencing Complex, pubmed-meshheading:19812547-Testis
pubmed:year
2009
pubmed:articleTitle
A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila.
pubmed:affiliation
Keio University School of Medicine, Tokyo 160-8582, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't