Linked Life Data

19812358

Source:http://linkedlifedata.com/resource/pubmed/id/19812358

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-11-25
pubmed:abstractText
Sulfate (SO(4)(2-)) is the second most abundant anion in seawater (SW), and excretion of excess SO(4)(2-) from ingested SW is essential for marine fish to survive. Marine teleosts excrete SO(4)(2-) via the urine produced in the kidney. The SO(4)(2-) transporter that secretes and concentrates SO(4)(2-) in the urine has not previously been identified. Here, we have identified and characterized candidates for the long-sought transporters. Using sequences from the fugu database, we have cloned cDNA fragments of all transporters belonging to the Slc13 and Slc26 families from mefugu (Takifugu obscurus). We compared Slc13 and Slc26 mRNA expression in the kidney between freshwater (FW) and SW mefugu. Among 14 clones examined, the expression of a Slc26a6 paralog (mfSlc26a6A) was the most upregulated (30-fold) in the kidney of SW mefugu. Electrophysiological analyses of Xenopus oocytes expressing mfSlc26a6A, mfSlc26a6B, and mouse Slc26a6 (mSlc26a6) demonstrated that all transporters mediate electrogenic Cl(-)/SO(4)(2-), Cl(-)/oxalate(2-), and Cl(-)/nHCO(3)(-) exchanges and electroneutral Cl(-)/formate(-) exchange. Two-electrode voltage-clamp experiments demonstrated that the SO(4)(2-)-elicited currents of mfSlc26a6A is quite large (approximately 35 microA at +60 mV) and 50- to 200-fold higher than those of mfSlc26a6B and mSlc26a6. Conversely, the currents elicited by oxalate and HCO(3)(-) are almost identical among mfSlc26a6A, mfSlc26a6B, and mSlc26a6. Kinetic analysis revealed that mfSlc26a6A has the highest SO(4)(2-) affinity as well as capacity. Immunohistochemical analyses demonstrated that mfSlc26a6A localizes to the apical (brush-border) region of the proximal tubules. Together, these findings suggest that mfSlc26a6A is the most likely candidate for the major apical SO(4)(2-) transporter that mediates SO(4)(2-) secretion in the kidney of marine teleosts.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1522-1490
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
297
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
R1647-59
pubmed:dateRevised
2011-3-3
pubmed:meshHeading
pubmed-meshheading:19812358-Adaptation, Physiological, pubmed-meshheading:19812358-Animals, pubmed-meshheading:19812358-Antiporters, pubmed-meshheading:19812358-Bicarbonates, pubmed-meshheading:19812358-Biological Transport, pubmed-meshheading:19812358-Blotting, Northern, pubmed-meshheading:19812358-Chlorides, pubmed-meshheading:19812358-Cloning, Molecular, pubmed-meshheading:19812358-Data Mining, pubmed-meshheading:19812358-Databases, Genetic, pubmed-meshheading:19812358-Female, pubmed-meshheading:19812358-Fish Proteins, pubmed-meshheading:19812358-Formic Acids, pubmed-meshheading:19812358-Fresh Water, pubmed-meshheading:19812358-Genetic Association Studies, pubmed-meshheading:19812358-Immunohistochemistry, pubmed-meshheading:19812358-Kidney, pubmed-meshheading:19812358-Kinetics, pubmed-meshheading:19812358-Membrane Potentials, pubmed-meshheading:19812358-Membrane Transport Proteins, pubmed-meshheading:19812358-Mice, pubmed-meshheading:19812358-Oocytes, pubmed-meshheading:19812358-Oxalic Acid, pubmed-meshheading:19812358-RNA, Messenger, pubmed-meshheading:19812358-Seawater, pubmed-meshheading:19812358-Sulfates, pubmed-meshheading:19812358-Takifugu, pubmed-meshheading:19812358-Xenopus
pubmed:year
2009
pubmed:articleTitle
Identification of renal transporters involved in sulfate excretion in marine teleost fish.
pubmed:affiliation
Department of Biological Sciences, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural