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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2009-11-13
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pubmed:abstractText |
Immunofluorescence is the basis for many techniques used to quantify phenomena in neuroscience research, in both normal and pathological tissue. Autofluorescence (non-specific, broad spectrum background fluorescence) is an unfortunate consequence of damage to brain tissue. Damage-induced autofluorescence potentially confounds analyses of tissue labeled with fluorescent markers in many experiments. This is especially problematic in protocols that utilize co-localization methods such as BrdU/NeuN in which autofluorescence might lead to overestimates of the number of double-labeled cells. Techniques to reduce autofluorescence are variable and relatively ineffective in damaged brain tissue. Here we show using confocal microscopy that damage-induced autofluorescence does not co-localize with the nuclear specific markers DAPI or Hoechst. Further co-localization of nuclear markers such as Ki67 or BrdU/NeuN with DAPI or Hoechst should serve to help discriminate between intended and spurious fluorescent signal.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1872-678X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
45-9
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pubmed:meshHeading |
pubmed-meshheading:19747946-Animals,
pubmed-meshheading:19747946-Antibodies,
pubmed-meshheading:19747946-Artifacts,
pubmed-meshheading:19747946-Benzimidazoles,
pubmed-meshheading:19747946-Biological Markers,
pubmed-meshheading:19747946-Brain Infarction,
pubmed-meshheading:19747946-Cell Nucleus,
pubmed-meshheading:19747946-Dentate Gyrus,
pubmed-meshheading:19747946-Disease Models, Animal,
pubmed-meshheading:19747946-False Positive Reactions,
pubmed-meshheading:19747946-Fluorescence,
pubmed-meshheading:19747946-Fluorescent Dyes,
pubmed-meshheading:19747946-Image Processing, Computer-Assisted,
pubmed-meshheading:19747946-Immunohistochemistry,
pubmed-meshheading:19747946-Indoles,
pubmed-meshheading:19747946-Ki-67 Antigen,
pubmed-meshheading:19747946-Male,
pubmed-meshheading:19747946-Microscopy, Confocal,
pubmed-meshheading:19747946-Neurons,
pubmed-meshheading:19747946-Predictive Value of Tests,
pubmed-meshheading:19747946-Rats,
pubmed-meshheading:19747946-Rats, Long-Evans,
pubmed-meshheading:19747946-Sensitivity and Specificity
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pubmed:year |
2009
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pubmed:articleTitle |
A novel method for reliable nuclear antibody detection in tissue with high levels of pathology-induced autofluorescence.
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pubmed:affiliation |
The Canadian Centre for Behavioural Neuroscience, Department of Neuroscience, University of Lethbridge, Lethbridge, Canada. simon.spanswick@uleth.ca
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pubmed:publicationType |
Journal Article
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