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rdf:type |
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lifeskim:mentions |
umls-concept:C0007102,
umls-concept:C0017262,
umls-concept:C0020281,
umls-concept:C0059438,
umls-concept:C0086982,
umls-concept:C0162638,
umls-concept:C0185117,
umls-concept:C0334227,
umls-concept:C0599946,
umls-concept:C1150553,
umls-concept:C1325180,
umls-concept:C1325181,
umls-concept:C1418898,
umls-concept:C1565860,
umls-concept:C1704263,
umls-concept:C1705323,
umls-concept:C2587213,
umls-concept:C2911684
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pubmed:dateCreated |
2009-9-2
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pubmed:abstractText |
This study investigated the apoptotic regulation by green tea catechin epigallcatechin-3-gallate (EGCG) on colon cancer cells in the presence of low-dose H(2)O(2) known to exert the activation of signal pathways leading to cell proliferation. In the presence of low-dose H(2)O(2), EGCG induced apoptosis and abolished the cell-proliferative effect exhibited by low-dose H(2)O(2). This reduction of growth was accompanied by an activation of AMP-activated kinase (AMPK), a decrease in cyclooxygenase-2 (COX-2) expression and prostaglandin E(2) (PGE(2)) levels, and the induction of apoptotic markers such as p53 and poly(ADP-ribose) polymerase (PARP) cleavage. The low-dose H(2)O(2) stimulated COX-2 expression, and treating cells with synthetic AMPK activator AICAR (5-aminoimiazole-4-carboxamide-1-beta-d-ribofuranoside) resulted in greater suppression of COX-2 expression and PGE(2). By treating cells with high concentrations of the reactive oxygen species (ROS) scavenger NAC (N-acetyl-1-cysteine), the apoptotic effect of EGCG was abolished and led to suppression of AMPK and COX-2, indicating that the liberation of excessive ROS might be the upstream signal of the AMPK-COX-2 signaling pathway even in the presence of low-dose H(2)O(2).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AICA ribonucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/AMP-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Aminoimidazole Carboxamide,
http://linkedlifedata.com/resource/pubmed/chemical/Catechin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radical Scavengers,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidants,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Tea,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/epigallocatechin gallate
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1749-6632
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
1171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
538-44
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pubmed:meshHeading |
pubmed-meshheading:19723101-AMP-Activated Protein Kinases,
pubmed-meshheading:19723101-Acetylcysteine,
pubmed-meshheading:19723101-Aminoimidazole Carboxamide,
pubmed-meshheading:19723101-Apoptosis,
pubmed-meshheading:19723101-Blotting, Western,
pubmed-meshheading:19723101-Catechin,
pubmed-meshheading:19723101-Cell Proliferation,
pubmed-meshheading:19723101-Colonic Neoplasms,
pubmed-meshheading:19723101-Cyclooxygenase 2,
pubmed-meshheading:19723101-Dinoprostone,
pubmed-meshheading:19723101-Dose-Response Relationship, Drug,
pubmed-meshheading:19723101-Enzyme Activation,
pubmed-meshheading:19723101-Free Radical Scavengers,
pubmed-meshheading:19723101-HT29 Cells,
pubmed-meshheading:19723101-Humans,
pubmed-meshheading:19723101-Hydrogen Peroxide,
pubmed-meshheading:19723101-Oxidants,
pubmed-meshheading:19723101-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:19723101-Reactive Oxygen Species,
pubmed-meshheading:19723101-Ribonucleotides,
pubmed-meshheading:19723101-Signal Transduction,
pubmed-meshheading:19723101-Tea,
pubmed-meshheading:19723101-Tumor Suppressor Protein p53
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pubmed:year |
2009
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pubmed:articleTitle |
Green tea catechin controls apoptosis in colon cancer cells by attenuation of H2O2-stimulated COX-2 expression via the AMPK signaling pathway at low-dose H2O2.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Medical Research Center for Bioreaction to Reactive Oxygen Species, Kyung Hee University College of Medicine, Seoul, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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