|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0003765,
umls-concept:C0013879,
umls-concept:C0017262,
umls-concept:C0021920,
umls-concept:C0024337,
umls-concept:C0185117,
umls-concept:C0291181,
umls-concept:C0441712,
umls-concept:C0596902,
umls-concept:C0851285,
umls-concept:C1314939,
umls-concept:C1335290,
umls-concept:C1442751,
umls-concept:C2911684
|
|
pubmed:issue |
47
|
|
pubmed:dateCreated |
2009-11-16
|
|
pubmed:abstractText |
Expression of the arginine/lysine transporter Cat-1 is highly induced in proliferating and stressed cells via mechanisms that include transcriptional activation. A bifunctional INE (intronic element) within the first intron of the Cat-1 gene was identified and characterized in this study. The INE had high sequence homology to an amino acid response element and was shown to act as a transcriptional enhancer in unstressed cells by binding the transcription factor, purine-rich element binding protein A (Pur alpha). During endoplasmic reticulum stress, binding of Pur alpha to the INE decreased; the element acted as a positive regulator in early stress by binding of the transcription factor ATF4 and as a negative regulator in prolonged stress by binding the stress-induced C/EBP family member, CHOP. We conclude that transcriptional control of the Cat-1 gene is tightly controlled by multiple cis-DNA elements, contributing to regulation of cationic amino acid transport for cell growth and proliferation. In addition, we propose that genes may use stress-response elements such as the INE to support basal expression in the absence of stress.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATF4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Cationic Amino Acid Transporter 1,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PURA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pura protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Puralpha protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor CHOP,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
1083-351X
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
20
|
|
pubmed:volume |
284
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
32312-20
|
|
pubmed:dateRevised |
2011-3-3
|
|
pubmed:meshHeading |
pubmed-meshheading:19720825-Activating Transcription Factor 4,
pubmed-meshheading:19720825-Animals,
pubmed-meshheading:19720825-Cationic Amino Acid Transporter 1,
pubmed-meshheading:19720825-Cell Line, Tumor,
pubmed-meshheading:19720825-Cell Proliferation,
pubmed-meshheading:19720825-DNA,
pubmed-meshheading:19720825-DNA-Binding Proteins,
pubmed-meshheading:19720825-Endoplasmic Reticulum,
pubmed-meshheading:19720825-Enhancer Elements, Genetic,
pubmed-meshheading:19720825-Gene Expression Regulation,
pubmed-meshheading:19720825-Humans,
pubmed-meshheading:19720825-Introns,
pubmed-meshheading:19720825-Mice,
pubmed-meshheading:19720825-Nerve Tissue Proteins,
pubmed-meshheading:19720825-Rats,
pubmed-meshheading:19720825-Transcription Factor CHOP,
pubmed-meshheading:19720825-Transcription Factors
|
|
pubmed:year |
2009
|
|
pubmed:articleTitle |
A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Pur alpha).
|
|
pubmed:affiliation |
Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4954, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
