Linked Life Data

19696964

Source:http://linkedlifedata.com/resource/pubmed/id/19696964

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-9-18
pubmed:abstractText
Bovine serum albumin (BSA) is generally used in biomedical experiments. In the solution of some reagents, BSA is necessary to maintain the stability and concentration of the effective component. Therefore, the potential impact of BSA on experimental results should not be neglected when BSA is used. In this study, we observed that BSA induced significant upregulation of mRNA expression and release of pro-inflammatory cytokines, IL-1beta, and TNF-alpha, by N9 microglial cells. Our results suggest that the effects of BSA should be taken into account in experiments on microglia or the central nervous system when BSA is used. In light of the high similarity and homology among mammalian albumins, our findings also indicate that serum albumin may be a potent trigger of cytokine release by microglia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1590-3478
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
379-83
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Bovine serum albumin promotes IL-1beta and TNF-alpha secretion by N9 microglial cells.
pubmed:affiliation
Department of Neurosurgery, Southwest Hospital, The Third Military Medical University, Chongqing, 400038, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't