Linked Life Data

19692404

Source:http://linkedlifedata.com/resource/pubmed/id/19692404

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1674
pubmed:dateCreated
2009-9-29
pubmed:abstractText
Monochamus alternatus is the longicorn beetle notorious as a vector of the pinewood nematode that causes the pine wilt disease. When two populations of M. alternatus were subjected to diagnostic polymerase chain reaction (PCR) detection of four Wolbachia genes, only the ftsZ gene was detected from one of the populations. The Wolbachia ftsZ gene persisted even after larvae were fed with a tetracycline-containing diet for six weeks. The inheritance of the ftsZ gene was not maternal but biparental, exhibiting a typical Mendelian pattern. The ftsZ gene titres in homozygotic ftsZ(+) insects were nearly twice as high as those in heterozygotic ftsZ(+) insects. Exhaustive PCR surveys revealed that 31 and 30 of 214 Wolbachia genes examined were detected from the two insect populations, respectively. Many of these Wolbachia genes contained stop codon(s) and/or frame shift(s). Fluorescent in situ hybridization confirmed the location of the Wolbachia genes on an autosome. On the basis of these results, we conclude that a large Wolbachia genomic region has been transferred to and located on an autosome of M. alternatus. The discovery of massive gene transfer from Wolbachia to M. alternatus would provide further insights into the evolution and fate of laterally transferred endosymbiont genes in multicellular host organisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0962-8452
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3791-8
pubmed:dateRevised
2010-11-9
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Longicorn beetle that vectors pinewood nematode carries many Wolbachia genes on an autosome.
pubmed:affiliation
Tohoku Research Center, Forestry and Forest Product Research Institute, Morioka, Japan. taikawa@ffpri.affrc.go.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't