Linked Life Data

19672952

Source:http://linkedlifedata.com/resource/pubmed/id/19672952

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-11-25
pubmed:abstractText
Spectrins are major proteins in the cytoskeletal network of most cells. In Drosophila, beta(Heavy)-Spectrin encoded by the karst gene functions together with Crb during photoreceptor morphogenesis. However, the roles of two other Spectrins (alpha- and beta-Spectrins) in developing photoreceptor cells have not been studied. Here, we analyzed the effects of spectrin mutations on developing eyes to determine their roles in photoreceptor morphogenesis. We found that the Spectrins are dispensable for retinal differentiation in eye imaginal discs during larval stage. However, photoreceptors deficient in alpha- or beta-Spectrin display dramatic apical membrane expansions including Crb and show morphogenesis defects during pupal eye development, suggesting that alpha- and beta-Spectrins are specifically required for photoreceptor polarity during pupal eye development. Karst localizes apically, whereas beta-Spectrin is preferentially distributed in the basolateral region. We show that overexpression of beta-Spectrin causes a strong shrinkage of apical membrane domains, and loss of beta-Spectrin causes an expansion of apical domains, implying an antagonistic relationship between beta-Spectrin and Karst. These results indicate that Spectrins are required for controlling photoreceptor morphogenesis through the modulations of cell membrane domains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1526-968X
pubmed:author
pubmed:copyrightInfo
(c) 2009 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
744-50
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Membrane domain modulation by Spectrins in Drosophila photoreceptor morphogenesis.
pubmed:affiliation
Department of Biology, Baylor University, Waco, Texas 76798, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't